KDM4A Protein (DYKDDDDK Tag)
Quick Overview for KDM4A Protein (DYKDDDDK Tag) (ABIN2669705)
抗原
See all KDM4A 蛋白蛋白类型
宿主
资源
应用范围
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标记
- This KDM4A protein is labelled with DYKDDDDK Tag.
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产品特性
- Recombinant JMJD2A / KDM4A (accession number NP_055478.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 125.3 kDa.
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Lysine (K)-Specific Demethylase 4A (KDM4A) (AA 888-1023) protein (His tag)
KDM4A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Lysine (K)-Specific Demethylase 4A (KDM4A) protein (Myc-DYKDDDDK Tag)KDM4A 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Recombinant JMJD2A / KDM4A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me3 and H3K36me3 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 100 nM Recombinant JMJD2A / KDM4A protein, and 3.3 μM H3K9me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
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限制
- 仅限研究用
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注意事项
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))
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别名
- JMJD2A / KDM4A
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背景
- KDM4A (lysine (K)-specific demethylase 4A), also known as JMJD2A (Jumonji Domain Containing 2A) is a nuclear protein that functions as a trimethylation-specific histone demethylase that preferentially demethylates trimethylated lysine 9 (K9me3) and lysine 36 (K36me3) residues of histone H3, converting these trimethylated histone residues to dimethylated form. KDM4A has no activity for mono- and dimethylated H3K9 and H3K36. KDM4A functions as a transcriptional repressor, participating in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. An additional KDM4A isoform, KDM4A Isoform 2, that lacks the N-terminal demethylase domain is crucial for muscle differentiation in promoting transcriptional activation of the MyoG gene by directing the removal of repressive chromatin marks at its promoter.
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分子量
- 125.3 kDa
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途径
- Warburg Effect
抗原
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