Histone 3 Protein (H3K4me2)
Quick Overview for Histone 3 Protein (H3K4me2) (ABIN2669498)
抗原
See all Histone 3 (H3) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- H3K4me2
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产品特性
- Recombinant Xenopus laevis Histone H3 dimethyl Lys4 (H3K4me2) is produced in E. coli and purified using FPLC. The protein contains a substitution of cysteine to alanine at amino acid 110. Recombinant methylated histones are specifically methylated via a chemical alkylation reaction that introduces a methyl lysine analog (MLA). This specific chemical treatment enables the site and degree of methylation to be controlled precisely. Each methylation reaction is over 99 % complete, as verified by high-resolution ESI-TOF mass spectrometry. Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. The recombinant histone is >98 % pure by SDS-PAGE. The molecular weight of the recombinant histone is 15,289 Daltons.
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纯化方法
- Purified using FPLC
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Histone H3 (H3) (H3K18ac) protein
H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant The recombinant histone is >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. PC, S
Histone H3 (H3) (pSer10) proteinH3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant The recombinant histone is >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. PC, S
Histone H3 (H3) (pThr3) proteinH3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant The recombinant histone is >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. PC, S
Histone H3 (H3) (H3K9me3) proteinH3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant The recombinant histone is >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. PC, S
Histone H3 (H3) (H3K9me) proteinH3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant The recombinant histone is >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. PC, S
Histone H3 (H3) (H3R8me2a) proteinH3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant The recombinant histone is >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. PC, S
Histone H3 (H3) (H3K4me2) proteinH3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant The recombinant histone is >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. PC, S
Histone H3 (H3) (H3K9me2) proteinH3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant The recombinant histone is >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. PC, S
Histone H3 (H3) (H3K4me3) proteinH3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant The recombinant histone is >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. PC, S
Histone H3 (H3) (C110A) proteinH3 宿主: 非洲爪蟾 宿主: 大肠杆菌（E. Coli） Recombinant The recombinant histone is >98 % pure by SDS-PAGE. PC, S
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应用备注
- Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/mL. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.
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注意事项
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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储存条件
- -20 °C/-80 °C
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储存方法
- Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Histone 3 (H3) (Histone H3 (H3))
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别名
- Histone H3
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背景
- Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.
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分子量
- The molecular weight of the recombinant histone is 15,289 Daltons.
抗原
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