Histone 3 Protein (full length, H3K9me1, truncated) (His tag)
Our Local Distributor
北京 101111
Quick Overview for Histone 3 Protein (full length, H3K9me1, truncated) (His tag) (ABIN2669569)
抗原
See all Histone 3 (H3) 蛋白蛋白类型
宿主
资源
应用范围
纯度
-
-
产品特性
- H3K9me1, full length, truncated
-
标记
- This Histone 3 protein is labelled with His tag.
-
原理
- Recombinant Histone H3K9me1 (EPL)
-
产品特性
- Recombinant Histone H3 monomethyl Lys9 (H3K9me1) proteins are generated using expressed protein ligation (EPL) technology. Truncated human Histone H3.2 is produced in E. coli and purified using FPLC. The purified protein is subsequently ligated to an N-terminal histone tail peptide containing monomethyl lysine 9 via a native peptide bond. The full-length protein is then repurified prior to lyophilization. Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. The recombinant histone is >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. The molecular weight of the recombinant histone is 15,239 Daltons. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. Recombinant histones are used to analyze histone modifications, or to generate chromatin in vitro.
-
纯化方法
- purified using FPLC.
-
-
Histone H3 (H3) (C110A) protein (His tag)
H3 宿主: 非洲爪蟾 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE PC, S
Histone H3 (H3) (full length), (H3S10ph), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3T3ph), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3K14ac), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3K23ac), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3K18ac), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3K4me2), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3K4me3), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3R8me2a), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3K9me2), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
-
-
应用备注
- Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/mL. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.
-
缓冲液
- 25 μg supplied as lyophilized powder. Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/mL. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.
-
注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
-
-
- Histone 3 (H3) (Histone H3 (H3))
-
别名
- Histone H3
-
背景
-
Protein Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.
Short Description: Recombinant Histone H3 monomethyl Lys9 (H3K9me1) proteins are generated using expressed protein ligation (EPL) technology. Truncated human Histone H3.2 is produced in E. coli and purified using FPLC. The purified protein is subsequently ligated to a N-terminal histone tail peptide containing monomethyl lysine 9 via a native peptide bond. The full-length protein is then repurified prior to lyophilization. Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. The recombinant histone is >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. The molecular weight of the recombinant histone is 15,239 Daltons. To see a representative mass spectrum, please visit our website at www.activemotif.com and view the product details page.
-
分子量
- 15,239 Daltons
抗原
-
