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- 抗原 See all Annexin V (ANXA5) 蛋白
- Annexin V (ANXA5) (Annexin A5 (ANXA5))
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宿主
- 哺乳动物
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Annexin V protein is labelled with FITC.
- 应用范围
- Biochemical Assay (BCA)
- 产品特性
- Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer (cat. no. 422201) is recommended for use with Annexin V staining.Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. So, we recommend using our 7-AAD Viability Staining Solution (cat. no. 420403/420404) or Propidium Iodide Solution (cat. no. 421301). Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.
- 纯化方法
- The purified protein was conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
- 组件
- FITC Annexin V
- Top Product
- Discover our top product ANXA5 蛋白
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Annexin A5 (ANXA5) (AA 2-320) protein
ANXA5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Annexin A5 (ANXA5) (AA 1-319) protein (His-EGFP Tag)ANXA5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Annexin A5 (ANXA5) (AA 1-320) protein (GST tag)ANXA5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Annexin A5 (ANXA5) protein (FITC)ANXA5 宿主: Bacteria > 99 % (SDS-PAGE)
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 缓冲液
- Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from prolonged exposure to light. Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- The Annexin V solution should be stored undiluted between 2°C and 8°C.
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- 抗原
- Annexin V (ANXA5) (Annexin A5 (ANXA5))
- 别名
- Annexin V (ANXA5 产品)
- 别名
- anx Protein, anx5 Protein, ANX V Protein, anxa5 Protein, cb989 Protein, wu:fa98f06 Protein, wu:fj10f10 Protein, MGC89158 Protein, ANX5 Protein, ENX2 Protein, PP4 Protein, RPRGL3 Protein, Anx5 Protein, R74653 Protein, LC5 Protein, enx2 Protein, annexin A5 Protein, annexin A5b Protein, Annexin A5 Protein, annexin A5 L homeolog Protein, ANXA5 Protein, anxa5b Protein, anxa5 Protein, Anxa5 Protein, anxa5.L Protein
- 途径
- Apoptosis
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