WISP1 Protein (AA 23-367)
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Quick Overview for WISP1 Protein (AA 23-367) (ABIN2667610)
抗原
See all WISP1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 23-367
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内毒素水平
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Less than 0.1 ng per μg of protein.
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WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 23-367) protein (His tag)
WISP1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) (AA 1-280) protein (GST tag)WISP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0 - 3.0 μg/ml.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. Note: Slow to dissolve. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.
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缓冲液
- Lyophilized
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
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别名
- WISP-1
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背景
- WNT1 inducible signaling protein (WISP) is a protein subfamily that belongs to the connective tissue growth factor (CTGF) family. This family includes connective tissue growth factor (CTGF), cysteine-rich-61 (CYR61), and nephroblastoma overexpressed (NOV). CTGF is a family that is cysteine-rich, carries four conserved domains from N- to C-terminus: insulin-like growth factor binding protein (IGFBP) domain, von Willebrand type C repeats (vWC) domain, thrombospondin type 1 repeat (TSR) domain, and a C-terminal domain (CT) with a cysteine knot motif. CTGF family is comprised of several extracellular matrix associated proteins that contribute to cell adhesion, migration, and wound repair. CTGF family member WISP-1, like WISP-2, and WISP-3, is a secreted extracellular matrix associated signaling protein that plays an important role in vessel formation, and bone and cartilage development. WISP-1 stimulates mesenchymal cell proliferation and osteoblastic differentiation. It is a downstream regulator of Wnt/Frizzled signaling that mediates various developmental processes and thus was identified as an oncogene modulated by the Wnt-1-β -catenin pathway. It is also known that WISP-1 is involved in prevention of late stage p53-mediated cell death and elevates expression of antiapoptotic Bcl-xL. WISP-1 overexpression, on the other hand, is associated with tumor formation due to its critical roles in early stage cellular proliferation and survival through phosphatidylinositol-3-kinase (PI3-K) and protein kinase B (Akt) pathways.
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分子量
- The 345 amino acids recombinant protein has a predicted molecular mass of 38.1 kDa. The predicted N-terminal amino acid is Thr.
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途径
- WNT signaling, Growth Factor Binding
抗原
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