VEGFA Protein (AA 27-146)
Quick Overview for VEGFA Protein (AA 27-146) (ABIN2667604)
抗原
See all VEGFA 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 27-146
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过滤
- 0.22 μm filtered
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内毒素水平
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Endotoxin level is <0.1 EU/μg (<0.01 ng/μg) protein as determined by the LAL method.
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Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (His tag)
VEGFA 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (Fc Tag)VEGFA 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) (Active) protein (His tag,AVI tag,Biotin)VEGFA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-146) (Active) protein (His tag,AVI tag,Biotin)VEGFA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Vascular Endothelial Growth Factor A (VEGFA) proteinVEGFA 宿主: 大鼠 宿主: 酵母菌 Recombinant > 95 % as determined by SDS-PAGE
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (His tag)VEGFA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Vascular Endothelial Growth Factor A (VEGFA) proteinVEGFA 宿主: 小鼠 宿主: Yeast (Pichia pastoris) Recombinant > 95 % as determined by SDS-PAGE
Vascular Endothelial Growth Factor A (VEGFA) (AA 1-147) protein (GST tag)VEGFA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-146) protein (His tag)VEGFA 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS
Vascular Endothelial Growth Factor A (VEGFA) proteinVEGFA 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 = 1-4 ng/ml, corresponding to a specific activity of 1-0.25 x 106 units/mg, as determined by the dose dependent stimulation of HUVEC cells proliferation.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22μm filtered protein solution is in 5 mM Citric Acid, 5 mM NaH2PO4, 150 mM NaCl, pH 4.0.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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别名
- VEGF120
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背景
- VegfA belong to the VEGF family which has at least 7 members, VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, PlGF and snake venom-derived VEGFs such as T.f. (Trimeresurus flavoviridis) svVEGF. In humans, VEGF-A is highly expressed in most of the solid tumors generated in breast, lung, renal, colorectal and liver tissues. VEGF-A has strong vascular permeability activity, and significantly contributes to the formation of ascites tumors. VEGF can act as a direct proinflammatory mediator during the pathogenesis of RA, and protect rheumatoid synoviocytes from apoptosis, which contributes to synovial hyperplasia. VEGF is expressed in synovial macrophages and synovial fibroblasts in the synovial tissues of RA patients.
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分子量
- The 120 amino acid recombinant protein has a predicted molecular mass of approximately 14,071 Da. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 15kDa and the non-reduced protein migrates as a homodi
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途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原
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