Neurotrophin 4 Protein (NTF4) (AA 81-210)
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- 抗原 See all Neurotrophin 4 (NTF4) 蛋白
- Neurotrophin 4 (NTF4)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 81-210
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Western Blotting (WB)
- 纯度
- >98 % , as determined by SDS-PAGE and HPLC analysis.
- 内毒素水平
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Less than 0.1 ng per μg of protein.
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Neurotrophin 4 (NTF4) (AA 81-210) protein
NTF4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS - PAGE SDS
Neurotrophin 4 (NTF4) (AA 1-210) protein (GST tag)NTF4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Neurotrophin 4 (NTF4) (AA 111-209) protein (GST tag)NTF4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: The ED50, determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forbrain primary septal cell cultures, was found to be in the range of 20-50 ng/ml, corresponding to a specific activity of 2-5 x 104 units/mg.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.
- 缓冲液
- Lyophilized
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- 抗原
- Neurotrophin 4 (NTF4)
- 别名
- NT-4 (NTF4 产品)
- 别名
- NTF5 Protein, GLC10 Protein, GLC1O Protein, NT-4 Protein, NT-4/5 Protein, NT-5 Protein, NT4 Protein, NT5 Protein, NT4P Protein, Ntf5 Protein, 2900040K06Rik Protein, AI462899 Protein, NT4/5 Protein, Ntf-5 Protein, Ntf4 Protein, neurotrophin 4 Protein, neurotrophin 5 Protein, NTF4 Protein, Ntf4 Protein, ntf4 Protein, Ntf5 Protein
- 背景
- Neurotrophins are growth factors that modulate growth, differentiation, and survival of neurons. Structure related proteins include neuron growth factor (NGF), brain derived neurogenic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4/5 (NT-4). They are highly homologous and share a similar structure with a tertiary folded, cysteine rich "knot". Neurotrophins are critical to the development of the central and peripheral nervous systems and play important roles in injury induced neuron regeneration. NT-4 is encoded by the NTF4 gene. The name NT-4/5 arose from the uncertainties between neurotrophin-5 found in humans and neurotrophin-4 identified in Xenopus. Although NT-4 is structurally the most divergent neurotrophin, like BNDF NT-4 binds to receptor TrkB with high affinity and mediates similar functions with BDNF in neurogenesis. However, similar to NGF and NT-3 gene-knockouts, the disruption of BDNF function severely affects neuron development. However, NT-4 knockout mice show minor deficits, with nearly 50 % neuron loss in nodose-petrosal and geniculate ganglia. The double knockouts of BDNF and NT-4 present additive deficit phenotype compared to NT-4 knockout mice. It is thus suggested that there are two groups of downstream target cells through multiple coreceptors interaction of BDNF/TrkB and NT-4/TrkB signaling. There are also some exceptions of biological actions of NT-4 that are not parallel with BDNF function. NT-4, but not BDNF, is reported to promote survival of striatal neurons and is able to overturn spatial memory impairments in aged rats. NT-4 is also suggested to participate in stem cell differentiation toward oligodendrocyte progenitor cells in the brain.
- 分子量
- NT-4 recombinant protein is a noncovalent linked homodimer. The 130 amino acid recombinant protein has a predicted molecular mass of approximately 14 kDa as monomer, 28 kDa as dimer. The predicted N-terminal Amino acid is Gly.
- 途径
- RTK signaling
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