Neurotrophin 4 Protein (NTF4) (AA 81-210)
Quick Overview for Neurotrophin 4 Protein (NTF4) (AA 81-210) (ABIN2667564)
抗原
See all Neurotrophin 4 (NTF4) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 81-210
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内毒素水平
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Less than 0.1 ng per μg of protein.
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Neurotrophin 4 (NTF4) (AA 1-210) protein (GST tag)
NTF4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Neurotrophin 4 (NTF4) (AA 81-210) proteinNTF4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS - PAGE SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: The ED50, determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forbrain primary septal cell cultures, was found to be in the range of 20-50 ng/ml, corresponding to a specific activity of 2-5 x 104 units/mg.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.
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缓冲液
- Lyophilized
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- Neurotrophin 4 (NTF4)
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别名
- NT-4
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背景
- Neurotrophins are growth factors that modulate growth, differentiation, and survival of neurons. Structure related proteins include neuron growth factor (NGF), brain derived neurogenic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4/5 (NT-4). They are highly homologous and share a similar structure with a tertiary folded, cysteine rich "knot". Neurotrophins are critical to the development of the central and peripheral nervous systems and play important roles in injury induced neuron regeneration. NT-4 is encoded by the NTF4 gene. The name NT-4/5 arose from the uncertainties between neurotrophin-5 found in humans and neurotrophin-4 identified in Xenopus. Although NT-4 is structurally the most divergent neurotrophin, like BNDF NT-4 binds to receptor TrkB with high affinity and mediates similar functions with BDNF in neurogenesis. However, similar to NGF and NT-3 gene-knockouts, the disruption of BDNF function severely affects neuron development. However, NT-4 knockout mice show minor deficits, with nearly 50 % neuron loss in nodose-petrosal and geniculate ganglia. The double knockouts of BDNF and NT-4 present additive deficit phenotype compared to NT-4 knockout mice. It is thus suggested that there are two groups of downstream target cells through multiple coreceptors interaction of BDNF/TrkB and NT-4/TrkB signaling. There are also some exceptions of biological actions of NT-4 that are not parallel with BDNF function. NT-4, but not BDNF, is reported to promote survival of striatal neurons and is able to overturn spatial memory impairments in aged rats. NT-4 is also suggested to participate in stem cell differentiation toward oligodendrocyte progenitor cells in the brain.
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分子量
- NT-4 recombinant protein is a noncovalent linked homodimer. The 130 amino acid recombinant protein has a predicted molecular mass of approximately 14 kDa as monomer, 28 kDa as dimer. The predicted N-terminal Amino acid is Gly.
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途径
- RTK signaling
抗原
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