Neurotrophin 3 Protein (NTF3) (AA 139-257, N-Term)
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- 抗原 See all Neurotrophin 3 (NTF3) 蛋白
- Neurotrophin 3 (NTF3)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 139-257, N-Term
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Western Blotting (WB)
- 纯度
- >98 % , as determined by SDS-PAGE and HPLC analysis.
- 内毒素水平
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Less than 0.1 ng per μg of protein.
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- Discover our top product NTF3 蛋白
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Neurotrophin 3 (NTF3) (Active) protein
NTF3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Neurotrophin 3 (NTF3) (AA 1-257) protein (GST tag)NTF3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Neurotrophin 3 (NTF3) (AA 19-128) protein (GST tag)NTF3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found in the range of 20-50 ng/ml, corresponding to a specific activity of 2-5 x 104 units/mg.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.
- 缓冲液
- Lyophilized
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- 抗原
- Neurotrophin 3 (NTF3)
- 别名
- NT-3 (NTF3 产品)
- 别名
- HDNF Protein, NGF-2 Protein, NGF2 Protein, NT3 Protein, NT-3 Protein, wu:fl98d04 Protein, hdnf Protein, ngf-2 Protein, ngf2 Protein, nt-3 Protein, nt3 Protein, NTF3 Protein, AI316846 Protein, AI835689 Protein, Nt3 Protein, Ntf-3 Protein, neurotrophin 3 Protein, neurotrophin 3 S homeolog Protein, neurotrophin 3 L homeolog Protein, NTF3 Protein, ntf3 Protein, ntf3.S Protein, Ntf3 Protein, ntf3.L Protein
- 背景
- Neurotrophins are growth factors that modulate growth, differentiation, and survival of neurons. Structure related proteins include neuron growth factor (NGF), brain derived neurogenic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4/5. They are highly homologous and share a similar structure of a tertiary folded, cysteine rich "knot". Neurotrophins are critical to the development of the central and peripheral nervous system and they play important roles in injury induced neuron regeneration. NT-3 is encoded by the the NTF3 gene and shares high similarity among species including human, pig, rat, and mouse. It is the only neurotrophin that affects the development of the enteric nervous system. NT-3 can act on neuroblast/progenitor cells directly or synergistically with other neurotrophins. It can regulate growth and maturation of neurons in both peripheral and central nervous systems. NT-3 elicits its activity mainly through activation of p75, tropomyosin-related kinase receptors (Trk), TrkB, and TrkC. NT-3 is the only ligand for TrkC and studies indicate that neuron generation in myenteric and submucosal plexuses is blocked in mice with deficiency in NT-3/TrkC signaling. The expression of NT-3 is regulated by bone morphogenetic protein 2 (BMP-2) and bone morphogenetic protein 4 (BMP-4). Although the NT-3-TrkC signal transduction is known to lead cellular apoptosis in the absence of ligand, overexpression of NT-3 conversely disrupts the tumor-preventive control and results in tumor generation. Dysregulation of NT-3 is known to be related with neuroblastoma, Alzhiemer's, Huntington's, and Parkinson's Disease. Due to the broad cell poplulations affected by NT-3, the control of NT-3-TrkC pathway has become a target for therapeutical approach for neurogenic diseases.
- 分子量
- NT-3 recombinant protein is a noncovalent linked homodimer. The 120 amino acid recombinant protein has a predicted molecular mass of approximately 13.6 kDa as monomer, 26.5 kDa as dimer. The predicted N-terminal amino acid is Met.
- 途径
- RTK signaling, Neurotrophin Signaling Pathway
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