IL-4 Protein (AA 21-140)
Quick Overview for IL-4 Protein (AA 21-140) (ABIN2667527)
抗原
See all IL-4 (IL4) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 21-140
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内毒素水平
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Less than 0.1 ng per μg of protein.
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Interleukin 4 (IL4) (AA 25-153) protein
IL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 4 (IL4) (AA 25-153) protein (His tag)IL4 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 4 (IL4) (AA 25-153) protein (His-Avi Tag,Biotin)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 4 (IL4) (AA 25-153) protein (His-Avi Tag)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 4 (IL4) (AA 23-140) (Active) proteinIL4 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 4 (IL4) (AA 25-153) (Active) proteinIL4 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag,AVI tag,Biotin)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 4 (IL4) protein (His tag)IL4 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 4 (IL4) (AA 63-153) protein (GST tag)IL4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 4 (IL4) (AA 25-153) protein (mFc Tag)IL4 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5.0 x 105 units/mg as determined by the dose-dependent stimulation of the proliferation of murine HT-2 cells.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
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缓冲液
- Lyophilized, carrier-free.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- IL-4 (IL4) (Interleukin 4 (IL4))
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别名
- IL-4
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背景
- IL-4 is a member of the short four-helix bundle family of cytokines. IL-4 is produced by Th2 cells in response to antigen receptor engagement. Dendritic cells can prime naive CD4+ T cell, nevertheless, it has been described that dendritic cell mediated priming is insufficient for the development of Th2 cell-dependent immunity. Basophils have been identified as a source of IL-4 in the initial naive CD4+ T cell priming. These cells coexpress MHC class II and IL-4 following helminth infection. Therefore, murine basophils could present antigens to naive T cells, thus promoting Th2 cell differentiation during allergic and anti-parasitic immune responses. In addition, it was previously known that upon cross-linkage of the high affinity receptor for immunoglobulin E, IL-4 is produced by NK cells, mast cells, and basophils. Cells of hematopoietic stem cell origin express the type I receptor for IL-4 (IL-4Ra, γc), and cells of non-hematopoietic stem cell origin express type II receptor (IL-4Ra, IL-13Ra1). Type I receptor complexes are active in regulating Th2 development. In contrast, the type II receptor complex is active in regulating cells that mediate airway hypersensitivity and mucus secretion.
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分子量
- The 121 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 13.5 kDa. The predicted N-terminal amino acid is Met.
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
抗原
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