IL23 Protein (AA 1-196, AA 1-335)
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Quick Overview for IL23 Protein (AA 1-196, AA 1-335) (ABIN2667513)
抗原
See all IL23 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-196, AA 1-335
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Interleukin 23 (IL23) protein
IL23 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Interleukin 23 (IL23) protein (His tag)IL23 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Interleukin 23 (IL23) proteinIL23 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 23 (IL23) (Active) protein (His tag)IL23 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 ≤1.5 ng/ml, corresponding to a specific activity of ≥0.66 x 106 units/mg, as determined by mouse splenocytes IL-17A secretion induced by mIL-23 in a dose dependent manner.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in PBS
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- IL23 (Interleukin 23 (IL23))
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别名
- IL-23
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背景
- IL-23 is a member of the IL-6 family of cytokines, and it is constituted by two subunits, p19 and p40. The p19-p40 heterodimer is stabilized by a disulfide bond. The subunit p40 is shared by IL-23 and IL-12 cytokines. p19 mRNA is expressed in endothelial cells and polarized T cells, nevertheless, p40 is not expressed by these cells. Therefore, the availability of functional IL-23 is limited by the expression of p40 and not p19. IL-23 exerts its biological activities through the interaction with a heterodimeric receptor complex composed of IL-12Rb1 and IL-23R. IL-23 activates Janus kinase (JAK)/signal transducer and activator of transcription signaling molecules (STAT). JAK2 is constitutively associated with the IL-23R chain, and binding of IL-23 to its receptor leads to phosphorylation of STAT1, STAT3, STAT4, and STAT5.
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分子量
- The total predicted molecular weight is 55.4 kDa. The non-reduced protein migrates at approximately 60 kDa and the DTT-reduced protein produces two bands at approximately 19 kDa (Leu 20-Ala 196) and 40 kDa (Met 23-Ser 335) by SDS-PAGE.
抗原
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