IL-22 Protein (AA 34-179)
Quick Overview for IL-22 Protein (AA 34-179) (ABIN2667507)
抗原
See all IL-22 (IL22) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 34-179
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过滤
- 0.22 μm filtered
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内毒素水平
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Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.
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Interleukin 22 (IL22) (AA 34-179) protein (His tag)
IL22 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 22 (IL22) (AA 34-179) protein (His tag)IL22 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 22 (IL22) (AA 34-179) protein (His-Avi Tag,Biotin)IL22 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Interleukin 22 (IL22) (AA 34-179) (Active) protein (His tag,AVI tag,Biotin)IL22 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 22 (IL22) protein (mFc Tag)IL22 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 22 (IL22) (AA 27-172) protein (His tag)IL22 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 22 (IL22) (AA 34-179) (Active) protein (His tag)IL22 宿主: 人 宿主: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. WB, SDS, ELISA, Imm, AcA Active
Interleukin 22 (IL22) (AA 1-179) protein (GST tag)IL22 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 22 (IL22) (Active) proteinIL22 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: The ED50 is 0.062-0.177 ng/ml, corresponding to a specific activity of 1.61-0.56 X107 units/mg, as determined by a dose dependent stimulation of human Colo205 cells in production of IL-10.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- IL-22 (IL22) (Interleukin 22 (IL22))
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别名
- IL-22
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背景
- IL-22 is a cytokine structurally related to IL-10. It was originally identified in mouse as a gene induced by IL-9 in T cells and mast cells.IL-22 was initially designated as ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely Il-10, IL-19, IL-20, IL-24, IL-26, IL-28A, IL-28B and IL-29 (the later three also known as IFN-λ). Human IL-22 shares 79 % amino acid identity with mouse IL-22 and 25 % identity with human IL-10. IL-22 biological activity is initiated by the binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which share sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of T helper 17 lineage of CD4 (+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in deregulated inflammation observed in autoimmune diseases.
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分子量
- The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16,880 Da. The DTT-reduced protein migrates at approximately 15kDa and the non-reduced protein migrates at approximately 14kDa by SDS-PAGE.
抗原
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