IL-25 Protein (AA 33-177, N-Term)
Quick Overview for IL-25 Protein (AA 33-177, N-Term) (ABIN2667476)
抗原
See all IL-25 (IL25) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 33-177, N-Term
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内毒素水平
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Less than 0.1 ng per μg of protein.
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Interleukin 25 (IL25) (AA 33-177) protein (His tag)
IL25 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 25 (IL25) (AA 33-177) protein (His-Avi Tag,Biotin)IL25 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 25 (IL25) (Active) proteinIL25 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 25 (IL25) (AA 33-177) protein (Fc Tag)IL25 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 25 (IL25) (AA 17-169) protein (His tag)IL25 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 25 (IL25) (AA 33-177) protein (His tag)IL25 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 25 (IL25) (AA 1-177) protein (GST tag)IL25 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 25 (IL25) (Active) protein (Fc Tag)IL25 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
Interleukin 25 (IL25) proteinIL25 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Human IL-17E is able to induce IL-8 secretion in human PBMCs using a concentration range of 10 - 100 ng/ml. Note: Results will vary with PBMC donors.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.
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缓冲液
- Lyophilized
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- IL-25 (IL25) (Interleukin 25 (IL25))
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别名
- IL-17E
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背景
- IL-17E, also known as IL-25, promotes Th2 biased immune responses. This is in contrast to other IL-17 family members which promote Th1 and Th17 biased inflammation. IL-17E is an important mediator of allergic reactions and protection against intestinal parasites. During helminth infections and allergic reactions, IL-17E production is locally upregulated in intestinal cells, airway epithelial cells, atopic dermatitis skin lesions, local Th2 cells, eosinophils, and basophils. It binds to IL-17RB but also requires IL-17RA to exert its activity. IL-17E acts on a variety of cell types which respond with increased production of Th2 cytokines and reduced production of Th1 and Th17 cytokines. Airway IL-17E can be activated by MMP7, a protease that is upregulated in airway epithelium in response to allergen exposure. Cleaved IL-17E shows enhanced binding to IL-17RB and stronger induction of Th2 cytokines. The Th2 cytokines, in turn, trigger the expansion of Th2 memory cells and anti-inflammatory M2 macrophages, increased eosinophil mobilization and activation, and dendritic cell migration. These actions promote protective anti-helminth immune responses as well as allergic inflammation and airway hyperreactivity. IL-17E also promotes vascular endothelial cell proliferation and assembly into tubular structures. It also supports the integrity of the blood brain barrier and limits CD4+ T cell infiltration into the brain.
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分子量
- The 146 amino acid recombinant protein has a predicted molecular mass of approximately 17 kDa. Recombinant human IL-17E forms a non-disulfide-linked homodimer (34 kDa). The predicted N-terminal amino acid is Met.
抗原
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