FGF2 Protein (AA 144-288)
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- 抗原 See all FGF2 蛋白
- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 144-288
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Intracellular Flow Cytometry (ICFC)
- 纯度
- Purity is >98 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.
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Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) protein
FGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Fibroblast Growth Factor 2 (Basic) (FGF2) (Active) proteinFGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 135-288), (Isoform 1) (Active) proteinFGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (Active) proteinFGF2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) (Active) protein (AVI tag,His tag,Biotin)FGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by SDS-PAGE. Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (Active) protein (His tag)FGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 9-154) protein (His tag)FGF2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 189-288) protein (GST tag)FGF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: ED50 = 1 - 4 ng/ml, corresponding to a specific activity of 1 - 0.25 x 106 units/mg, as determined by the dose dependent stimulation of NIH/ 3T3 cell proliferation. The bioactivity is equivalent to competitor reported values.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
- 缓冲液
- 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2, 1 mM DTT.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- 抗原
- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
- 别名
- FGF-Basic (FGF2 产品)
- 别名
- BFGF Protein, FGF-2 Protein, FGFB Protein, HBGF-2 Protein, Fgf-2 Protein, Fgfb Protein, bFGF Protein, fibroblast growth factor 2 Protein, fibroblast growth factor 2 (basic) Protein, FGF2 Protein, Fgf2 Protein, fgf2 Protein
- 背景
- FGFb is a member of the fibroblast growth factor (FGF) family which includes 23 members. FGFb is expressed in almost all tissues and play important roles in a variety of normal and pathological processes, including development, wound healing and neoplastic transformation. FGFb is mitogenic for many cell types, both epithelial and mesenchymal. FGFb shows potent angiogenic activity and has been implicated in tumor angiogenesis (2). In prostate, bladder, and renal cancers, FGFb regulates the induction of metalloproteinases (MMP) that degrade extracellular matrix proteins, thus facilitating tumor metastasis (3). FGFb binds to a family of four distinct, high affinity tyrosine kinase receptors, designated FGFR-1 to -4 (4). In addition, FGFb binds to the ECM, and heparan sulfate (HS) is an essential and dynamic regulator of fibroblast growth factor (FGF) signaling. Two fundamentally different crystallographic models have been proposed to explain, at the molecular level, how HS/heparin enables FGF and FGF receptor (FGFR) to assemble into a functional dimer on the cell surface (5), although there is controversy regarding the exact manner by which this occurs.
- 分子量
- The 145 amino acid recombinant protein has a predicted molecular mass of 16,310 Da. The DTT-reduced and non-reduced protein migrate at approximately 18kDa by SDS-PAGE. This protein may or may not contain an N-terminal methionine.
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins
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