IL-11 Protein (AA 20-199)
Quick Overview for IL-11 Protein (AA 20-199) (ABIN2666948)
抗原
See all IL-11 (IL11) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 20-199
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Interleukin 11 (IL11) (AA 22-199) protein (His tag)
IL11 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 11 (IL11) (AA 22-199) protein (His tag)IL11 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 11 (IL11) (AA 22-199) (Active) protein (His tag)IL11 宿主: 人 宿主: 大肠杆菌（E. Coli） > 90 % AcA, CC Active
Interleukin 11 (IL11) (Active) proteinIL11 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 11 (IL11) protein (Fc Tag)IL11 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Interleukin 11 (IL11) (AA 22-199) protein (His tag)IL11 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 11 (IL11) (AA 1-199) protein (GST tag)IL11 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 11 (IL11) (AA 22-199) protein (Fc Tag)IL11 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 11 (IL11) proteinIL11 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Interleukin 11 (IL11) (AA 22-199) (Active) protein (His tag)IL11 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: The ED50 is 4-12 ng/ml, corresponding to a specific activity 8.3 x 104-2.5 x 105 units/mg, as determined by a dose-dependent stimulation of 7TD1 cells proliferation.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA, or FBS. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- IL-11 (IL11) (Interleukin 11 (IL11))
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别名
- IL-11
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背景
- IL-11, a member of the IL-6 family of cytokines, exerts a wide range of biological effects on various cell types including hematopoietic cells, hepatocytes, adipocytes, neurons, and osteoblasts. IL-11 works synergistically with other growth factors including SCF, IL-4, IL-3, IL-7, IL-12, IL-13, and GM-CSF to stimulate the proliferation of cells from several hematopoietic lineages. Binding of IL-11 to IL-11 receptor (IL-11R) induces membrane bound gp130 homodimerization and triggers STAT3 phosphorylation by JAK. IL-11 shares the common receptor subunit gp130 with IL-6, IL-27, LIF, OSM, CNTF, CT-1, CLC, and NP. Female mice deficient in IL-11R revealed an important role for IL-11 in embryonic implantation. In addition, IL-11 shows anti-inflammatory activity in models of inflammatory bowel disease, chemotherapy induced oral mucositis, and inflammatory arthritis. IL-11 and IL-13 are highly expressed in asthmatic airways (Th2 response), and IL-11 can inhibit Th1 responses and inhibits the production of Th1 cytokines such as IL-12 and shifts inflammation in Th2 direction. Elevated IL-11 expression is associated with tumor grade and invasion in gastric cancer. Recombinant human IL-11 has been clinically approved to improve platelet recovery after chemotherapy-induced thrombocytopenia.
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分子量
- The 180 amino acid recombinant protein has a predicted molecular mass of approximately 19.3 kDa. The DTT-reduced and non reduced proteins migrate at approximately 19.3 kDa by SDS-PAGE. The N-terminal amino acid is Valine.
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途径
- JAK/STAT Signaling, Negative Regulation of Hormone Secretion
抗原
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