CXCL14 Protein (AA 23-99)
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- 抗原 See all CXCL14 蛋白
- CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 23-99
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- > 98 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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- Discover our top product CXCL14 蛋白
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Chemokine (C-X-C Motif) Ligand 14 (CXCL14) (Active) protein
CXCL14 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) (AA 23-99) protein (His tag)CXCL14 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) (AA 35-111) protein (His-IF2DI Tag)CXCL14 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) (AA 1-111) protein (GST tag)CXCL14 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) proteinCXCL14 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Bioactivity was measured by its property to chemoattract PGE2 activated THP-1 cells in a dose dependent manner.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
- 缓冲液
- 0.22 μm filtered protein solution is in PBS.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- 抗原
- CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))
- 别名
- CXCL14 (CXCL14 产品)
- 别名
- BMAC Protein, BRAK Protein, KEC Protein, KS1 Protein, MIP-2g Protein, MIP2G Protein, NJAC Protein, SCYB14 Protein, 1110031L23Rik Protein, 1200006I23Rik Protein, AI414372 Protein, Kec Protein, MIP2gamma Protein, Scyb14 Protein, bolekine Protein, scyba Protein, fb67g04 Protein, wu:fb67g04 Protein, JSC Protein, bmac Protein, brak Protein, kec Protein, ks1 Protein, mip-2g Protein, mip2g Protein, njac Protein, scyb14 Protein, CXCL14 Protein, DKFZp469J175 Protein, C-X-C motif chemokine ligand 14 Protein, chemokine (C-X-C motif) ligand 14 Protein, chemokine (C-X-C motif) ligand 14 L homeolog Protein, CXCL14 Protein, Cxcl14 Protein, cxcl14 Protein, cxcl14.L Protein
- 背景
- Mouse CXCL14 was identified from an EST database of a murine immature keratinocyte cDNA library. mRNA expression in mouse tissues showed high levels in brain, ovary, lung, and muscle. CXCL14 is well conserved between humans, birds, frogs and fish at the amino acid level, and human and mouse CXCL14 share 95 % identity. Original studies showed a decrease in the expression of CXCL14 in head and neck squamous cell carcinoma (HNSCC) and cervical squamous cell carcinoma (SCCs) tumors, and the induction of expression of CXCL14 in human oral carcinoma prevented tumor growth of these cells in vivo. Therefore, it was suggested that CXCL14 possesses tumor suppressing function, and it was speculated that CXCL14 secreted by stromal cells chemoattracts iDCs (immature DCs) and NK cells that activate the immune response against tumor cells. Most recent data showed that epigenetic silencing of CXCL5, CXCL12, and CXCL14 occurs in 75 % of primary lung adenocarcinomas. Consequently, the low expression of CXCL14 in tumors might allow them to escape immune surveillance. Opposite of HNSCC and SCCs tumors, prostate, pancreatic, and colorectal cancers show high expression of CXCL14, inducing growth and invasiveness of pancreatic and breast cancer cells.
- 分子量
- The 77 amino acid recombinant protein has a predicted molecular mass of approximately 9.5 kDa. The DTT-reduced and non-reduced protein migrate at approximately 13 and 14 kDa by SDS-PAGE respectively. The N-terminal amino acid is Met.
- 途径
- Autophagy
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