CXCL14 Protein (AA 35-111)
Quick Overview for CXCL14 Protein (AA 35-111) (ABIN2666914)
抗原
See all CXCL14 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 35-111
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Chemokine (C-X-C Motif) Ligand 14 (CXCL14) (Active) protein
CXCL14 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) (AA 35-111) protein (His-IF2DI Tag)CXCL14 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) (AA 23-99) protein (His tag)CXCL14 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) (AA 1-111) protein (GST tag)CXCL14 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) proteinCXCL14 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Bioactivity was measured by its property to chemoattract PGE2 activated THP-1 cells in a dose dependent manner.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))
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别名
- CXCL14
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背景
- CXCL14 was initially cloned from breast and kidney cells, and it is expressed at high levels and ubiquitously in normal tissue. CXCL14 is well conserved between humans, birds, frogs and fish at the amino acid level, and human and mouse CXCL14 share 95 % identity. Original studies showed a decrease in the expression of CXCL14 in head and neck squamous cell carcinoma (HNSCC) and cervical squamous cell carcinoma (SCCs) tumors, and the induction of expression of CXCL14 in human oral carcinoma prevents tumor growth of these cells in vivo. Therefore, it has been suggested that CXCL14 possesses tumor suppressing function, and it has been speculated that CXCL14 secreted by stromal cells chemoattracts iDCs (immature dendritic cells) and NK cells that activate the immune response against tumor cells. Most recent data showed that epigenetic silencing of CXCL5, CXCL12, and CXCL14 occurs in 75 % of primary lung adenocarcinomas. Consequently, the low expression of CXCL14 in tumors might allow them to escape immune surveillance. Opposite of HNSCC and SCCs tumors, prostate, pancreatic, and colorectal cancers show high expression of CXCL14, inducing growth and invasiveness of pancreatic and breast cancer cells.
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分子量
- The 77 amino acid recombinant protein has a predicted molecular mass of approximately 9.5 kDa. The DTT-reduced and non-reduced protein migrate at approximately 13 and 14 kDa by SDS-PAGE respectively. The N-terminal amino acid is Met.
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途径
- Autophagy
抗原
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