CCL20 Protein (Ala27, Met96)
Quick Overview for CCL20 Protein (Ala27, Met96) (ABIN2666889)
抗原
See all CCL20 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- Ala27, Met96
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Chemokine (C-C Motif) Ligand 20 (CCL20) protein (His tag)
CCL20 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Chemokine (C-C Motif) Ligand 20 (CCL20) (AA 27-95) protein (GST tag)CCL20 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Bioactivity was measured by its property to chemoattract BaF3-hCCR6 transfectants in a dose dependent manner.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
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别名
- CCL20
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背景
- During infections, CCL20 expression is stimulated in epithelial cells, and immature CCR6+ DC are recruited. Thereafter, CCR6 is downregulated during dendritic cell (DC) maturation, and these migrate to the lymphoid node under the expression of CCR7. CCL20 is the only ligand for CCR6. Nevertheless, mouse and human β-defensin-1 and -2, small cationic antimicrobial peptides, bind and activate CCR6. Contrarily, these β-defensins have lower affinities for CCR6 than CCL20. Also, human CCL20 has potent antimicrobial activity. CCL20 and CCR6 play an important role in the pathogenesis of encephalomyelitis and psoriasis (chronic inflammatory skin disorder) in animal models. CCL20 is a chemokine abundant in psoriatic plaques characterized by epidermal hyperproliferation and immunocyte infiltration. CCL20 production from keratinocytes could be an initial trigger for Th17 activation and a key component in maintaining the self-perpetuating cycle of Th17 activation in psoriasis.
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分子量
- The 70 amino acid recombinant protein has a predicted molecular mass of approximately 8.0 kDa. The DTT-reduced protein migrates at approximately 11 kDa and non-reduced protein at approximately 13 kDa by SDS-PAGE. The N-terminal amino acid is Ala.
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途径
- The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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