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Mesothelin Protein (MSLN) (AA 296-580, C-Term)

Name: Mesothelin Protein (MSLN) (AA 296-580, C-Term)
SKU: ABIN2666849
Availability: OutOfStock

This Recombinant Mesothelin protein is expressed in HEK-293 Cells.

产品编号 ABIN2666849

发货至: 中国

Quick Overview for Mesothelin Protein (MSLN) (AA 296-580, C-Term) (ABIN2666849)

抗原

See all Mesothelin (MSLN) 蛋白

Mesothelin (MSLN)

蛋白类型

Recombinant

生物活性

Active

宿主

人

资源

HEK-293 Cells

应用范围

Flow Cytometry (FACS)

纯度

> 95 % , as determined by Coomassie stained SDS-PAGE.

Discover our top product MSLN 蛋白

产品特性

AA 296-580, C-Term

过滤

0.22 μm filtered

内毒素水平

Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Product

表达系统

Conjugate

Origin

价格从

Mesothelin Protein (MSLN) (AA 1-375) (His tag)

表达系统 HEK-293 Cells

Conjugate His tag

Origin Human

价格从 136,947.45 ¥

Mesothelin Protein (MSLN) (AA 1-375) (Strep Tag)

表达系统 Cell-free protein synthesis (CFPS)

Conjugate Strep Tag

Origin Human

价格从 172,528.32 ¥

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Mesothelin (MSLN) (AA 296-580) protein (Fc Tag)
MSLN 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Mesothelin (MSLN) (AA 296-580) protein (Fc-Avi Tag,Biotin)
MSLN 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Mesothelin (MSLN) (AA 296-580) protein (Fc Tag)
MSLN 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE

Mesothelin (MSLN) (AA 296-580) protein (His-Avi Tag)
MSLN 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Mesothelin (MSLN) (AA 406-690) protein (His tag)
MSLN 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Mesothelin (MSLN) (AA 296-580) protein (mFc-His Tag)
MSLN 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. WB

Mesothelin (MSLN) (AA 406-690) protein (His tag,Biotin)
MSLN 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Mesothelin (MSLN) (AA 296-580) protein (His-Avi Tag,Biotin)
MSLN 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Mesothelin (MSLN) (AA 1-375) protein (His tag)
custom-made MSLN 宿主: 小鼠宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Mesothelin (MSLN) (AA 1-375) protein (His tag)
custom-made MSLN 宿主: 人宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

应用备注

Optimal working dilution should be determined by the investigator.

说明

Biological activity: ED50 = 50-300 ng/ml, corresponding to a specific activity of 0.33-2.0 x 104 units/mg, as determined by the dose dependent binding to CA125/MUC16 using a functional ELISA.

限制

仅限研究用
状态

Liquid

溶解方式

For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

缓冲液

0.22 μm filtered protein solution is in PBS, pH 7.2.

注意事项

Avoid repeated freeze/thaw cycles.

储存条件

-20 °C

储存方法

Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
抗原

Mesothelin (MSLN)

别名

Mesothelin

背景

Mesothelin (MSLN) was initially cloned from a library of human pancreatic cancer cells. It was also identified by a monoclonal antibody, MAb K1, which recognized a glycoprotein present on the surface of mesothelial cells. The human MSLN gene encodes a ~71 kD precursor protein of 622 amino acids. The C-terminal glycosylphosphatidylinositol (GPI) anchor addition signal is removed (cleavage site: Ser598) and the latter replaced with a GPI anchor. The MSLN precursor is cleaved by furin at Arg295 into two products, a ~31 kD mature megakaryocyte potentiating factor (MPF residues Ser34 - Arg286) and a ~40 kD GPI-anchored membrane-bound mature mesothelin starting from Glu296. The 40 kD GPI-anchored mesothelin can be released by treatment with phosphatidylinositol-specific phospholipase C. Soluble mesothelin specifically binds to ovarian carcinoma cell line OVCAR-3, mediated by the cancer antigen 125 (CA125), a mucin-like glycoprotein on the surface of tumor cells. CA125 and mesothelin are co-expressed in advanced grade ovarian adenocarcinoma, and it has been speculated that CA125 might contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to mesothelin. Also, mesothelin and CA125/mucin 16 (CA125/MUC16) are co-overexpressed at the invading edges of pancreatic cancer tissues, and their expression correlates with poor survival rates. Mesothelin is highly expressed in several human cancers including malignant mesothelioma, pancreatic, ovarian, cholangiocarcinoma, and lung adenocarcinoma.

分子量

The 291 amino acid recombinant protein has a predicted molecular mass of approximately 32.9 kDa. The DTT-reduced and non-reduced protein migrate at approximately 70 kDa and 150 kDa by SDS-PAGE respectively. The N-terminal amino acid is Glu.

途径

EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process