Mesothelin Protein (MSLN) (AA 296-580) (Biotin,His-Avi Tag)
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Quick Overview for Mesothelin Protein (MSLN) (AA 296-580) (Biotin,His-Avi Tag) (ABIN7275286)
抗原
See all Mesothelin (MSLN) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 296-580
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标记
- This Mesothelin protein is labelled with Biotin,His-Avi Tag.
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原理
- Biotinylated Human MSLN/Mesothelin Protein
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序列
- Glu296-Gly580
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产品特性
- Recombinant Biotinylated Human MSLN/Mesothelin Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu296-Gly580.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Anti-MSLN Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human MSLN, His Tag with the EC50 of 13.7ng/ml determined by ELISA.,Biotinylated Human MSLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human MSLN was greater than 95% as determined by SEC-HPLC.
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 150,642.20 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 145,985.55 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Mesothelin (MSLN) (AA 306-590) (Active) protein (hFc Tag)
MSLN 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Mesothelin (MSLN) (AA 296-580) (Active) protein (hFc-Avi Tag,Biotin)MSLN 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Mesothelin (MSLN) (AA 296-580) (Active) protein (hFc Tag)MSLN 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Mesothelin (MSLN) (AA 296-580) (Active) protein (His-Avi Tag)MSLN 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Mesothelin (MSLN) (AA 296-580) (Active) protein (His tag)MSLN 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Mesothelin (MSLN) (AA 296-580) (Active) protein (Biotin,His tag)MSLN 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Mesothelin (MSLN) (AA 296-580) protein (His tag,mFc Tag)MSLN 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. IA, ADA, AM, ScA
Mesothelin (MSLN) (AA 1-375) protein (His tag)custom-made MSLN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Mesothelin (MSLN) (AA 1-375) protein (His tag)custom-made MSLN 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Mesothelin (MSLN) (AA 1-621) protein (GST tag)MSLN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- Mesothelin (MSLN)
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别名
- MSLN
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背景
- Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31- kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
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分子量
- 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
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UniProt
- Q13421
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途径
- EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process
抗原
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