IL-25 Protein (AA 17-169)
Quick Overview for IL-25 Protein (AA 17-169) (ABIN2666841)
抗原
See all IL-25 (IL25) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 17-169
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Interleukin 25 (IL25) (AA 33-177) protein (His tag)
IL25 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 25 (IL25) (AA 33-177) protein (His-Avi Tag,Biotin)IL25 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 25 (IL25) (Active) proteinIL25 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 25 (IL25) (AA 33-177) protein (Fc Tag)IL25 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 25 (IL25) (AA 17-169) protein (His tag)IL25 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 25 (IL25) (AA 33-177) protein (His tag)IL25 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 25 (IL25) (AA 1-177) protein (GST tag)IL25 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 25 (IL25) (Active) protein (Fc Tag)IL25 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
Interleukin 25 (IL25) proteinIL25 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Mouse IL-25 binds to recombinant mouse IL-17BR/Fc chimera in a functional ELISA.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
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缓冲液
- 0.22 μm filtered protein solution is in 20 mM NaHPO4 pH 6.0, 0.5 M NaCl.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- IL-25 (IL25) (Interleukin 25 (IL25))
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别名
- IL-25
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背景
- IL-25 (also known as IL-17E) is a member of the IL-17 family, which includes IL-17A, IL-17B, IL-17C, IL-17F, and IL-17A/F. The primary sequence homology differs between family members, with mouse IL-17A and IL-17F having the highest homology (49 % ), while mouse IL-17A and IL-25 have the lowest (28 %). IL-25 induces gene expression of IL-4, IL-5, and IL-13 in multiple tissues, and this induction results in T helper 2 (TH2)-type immune responses, this is characterized by an increase in serum IgE levels, pathologic changes in the lungs and digestive tract with eosinophilic infiltrates, an increase in mucus production, epithelial cell hyperplasia, and overall amplified allergic inflammation. IL-25 shares the receptor IL-17RB with IL-17B, although it binds with much higher affinity than IL-17B.
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分子量
- The 153 amino acid recombinant protein has a predicted molecular mass of 17.6 kDa. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 20 kDa by SDS-PAGE. The non-reduced protein migrates as a homodimer,
抗原
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