C-Type Lectin Domain Family 1, Member B (CLEC1B) (AA 157-229), (C-Term) (Active) protein (Fc Tag)
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- 抗原 See all C-Type Lectin Domain Family 1, Member B (CLEC1B) 蛋白
- C-Type Lectin Domain Family 1, Member B (CLEC1B)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 157-229, C-Term
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- Fc Tag
- 应用范围
- Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 1.0 EU per μg of protein as determine by the LAL method.
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- Discover our top product CLEC1B 蛋白
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C-Type Lectin Domain Family 1, Member B (CLEC1B) (AA 1-196) protein (GST tag)
CLEC1B 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
C-Type Lectin Domain Family 1, Member B (CLEC1B) (AA 128-229) protein (GST tag)CLEC1B 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
C-Type Lectin Domain Family 1, Member B (CLEC1B) (AA 1-229) protein (Strep Tag)Crystallography grade CLEC1B 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
C-Type Lectin Domain Family 1, Member B (CLEC1B) (AA 55-229) protein (His tag)Crystallography grade CLEC1B 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
C-Type Lectin Domain Family 1, Member B (CLEC1B) (AA 53-229) protein (His tag)Crystallography grade CLEC1B 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
C-Type Lectin Domain Family 1, Member B (CLEC1B) (AA 1-229) protein (Strep Tag)Crystallography grade CLEC1B 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Recombinant mouse CLEC2, when coated on a plate (2 μg/ml, 100 μl/well) is able to bind biotinylated mouse GP38 in a dose dependent manner with an apparent kD <5.0 nM.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
- 浓度
- 200 μg/mL
- 缓冲液
- 0.22 μm filtered protein solution is in PBS.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- 抗原
- C-Type Lectin Domain Family 1, Member B (CLEC1B)
- 别名
- CLEC2 (CLEC1B 产品)
- 别名
- 1810061I13Rik Protein, CLEC2 Protein, CLEC2B Protein, PRO1384 Protein, QDED721 Protein, Clec-2 Protein, Clec2 Protein, CLEC1B Protein, RGD1563517 Protein, CLEC9A Protein, C-type lectin domain family 1 member B Protein, C-type lectin domain family 1, member b Protein, C-type lectin domain family 1, member B Protein, CLEC1B Protein, Clec1b Protein
- 物质类
- Viral Protein
- 背景
- CLEC2, also termed CLEC-1B, is a C-type lectin-like receptor. CLEC2 is highly expressed on resting platelets and megakaryocytes, and expressed at lower levels on several hematopoietic cells including monocytes, macrophages, dendritic cells, and granulocytes. The first ligand identified for CLEC2 was rhodocytin, snake venom toxin. Rhodocytin is a strong platelet activator, and this effect is mediated by CLEC2, which has a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (ITAM). Exposure of platelets to rhodocytin leads to tyrosine phosphorylation of the ITAM-like motif and Syk-dependent platelet activation. Podoplanin, also termed GP38 is an endogenous ligand of CLEC2. This molecule was found to be expressed on the surface of tumor cells and facilitates tumor metastasis by inducing platelet aggregation. Podoplanin is also expressed on endothelial cells, and mice deficient in this protein show abnormal patterns of lymphatic vessel formation. In addition, CLEC2 functions as an activating receptor on monoyctes and neutrophils to induce phagocytosis and proinflammatory cytokine production. CLEC2 enhances the expansion of TCR-stimulated T cells by increasing their survival through enhanced expression of anti-apoptotic proteins. Also, CLEC2 modulates the capacity of T cells to home to lymph nodes and stimulates natural killer cell mediated cytotoxicity.
- 分子量
- The 409 amino acid recombinant protein has a predicted molecular mass of approximately 46.7 kDa. The protein migrates at about 60 kDa by SDS-PAGE in DTT-reducing conditions and about 120 kDa in non-reducing conditions. The predicted N-terminal amino acid
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