CD70 Protein (AA 47-195)
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- 抗原 See all CD70 蛋白
- CD70 (CD70 Molecule (CD70))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 47-195
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宿主
- 小鼠
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资源
- CHO Cells
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- > 90 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.1 EU per μg of protein as determine by the LAL method.
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- Discover our top product CD70 蛋白
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CD70 Molecule (CD70) (AA 39-193) protein (Fc Tag)
CD70 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD70 Molecule (CD70) (AA 45-195) protein (Fc Tag)CD70 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD70 Molecule (CD70) (Trimer) protein (His tag)CD70 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
CD70 Molecule (CD70) (Trimer) protein (His tag,Biotin)CD70 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD70 Molecule (CD70) protein (mFc-His Tag)CD70 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD70 Molecule (CD70) (AA 39-194) protein (His tag)CD70 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,
CD70 Molecule (CD70) (AA 52-193) protein (His-DYKDDDDK-Strep II Tag)CD70 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
CD70 Molecule (CD70) (AA 52-193) (Active) protein (Fc Tag)CD70 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD70 Molecule (CD70) (AA 52-193) (Active) protein (DYKDDDDK Tag,His tag)CD70 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD70 Molecule (CD70) (AA 52-193) (Active) protein (His tag,AVI tag,DYKDDDDK Tag,Biotin)CD70 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Mouse CD27L induces proliferation of mouse T cells in the presence of anti-mouse CD3. The ED50 is 1.4 - 5.6 μg/mL.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
- 缓冲液
- 0.22 μm filtered protein solution is in PBS, pH 7.2.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- 抗原
- CD70 (CD70 Molecule (CD70))
- 别名
- CD27L (CD70 产品)
- 别名
- CD27LG Protein, Cd27l Protein, Tnfsf7 Protein, CD27L Protein, TNFSF7 Protein, CD70 antigen Protein, CD70 molecule Protein, Cd70 molecule Protein, Cd70 Protein, CD70 Protein
- 背景
- CD27L is a 50 kD type II transmembrane glycoprotein and member of the tumor necrosis factor superfamily. Mouse CD27L and its human counterpart share about 56 % amino acid sequence identity. The interaction between CD27L and CD27, a protein of the TNF receptor superfamily expressed on NK cells and subsets of T and B cells, provides costimulatory signals that are required for T cell proliferation, clonal expansion, and promotion of effector T cell formation. CD27L-CD27 interaction on mouse B cells on the other hand, has been shown to inhibit terminal differentiation of B cells into plasma cells. CD27L expression is induced by antigen receptor activation in B cells and at low levels in mouse T cells. CD27 interaction with recombinant soluble CD27L protein (sCD27L), has shown that sCD27L enhances IFN-γ secretion by CD56(bright) NK cells in the presence of IL-12, and these NK cells were able to suppress HCV replication. Also, CD8+ T cells stimulated with sCD27L in the presence of Ag showed enhanced proliferation and production of IL-2 and IFN-γ in vitro. In addition, administration of Ag and soluble CD27L induces expansion of Ag-specific CD8+ T cells in vivo. Data suggests that sCD27L is a potent stimulator of antiglioma immune responses that depend on CD8+ T cells, therefore, sCD27L might be an adjuvant for future immunotherapy trials for glioblastoma.
- 分子量
- The 170 amino acid recombinant protein has a predicted molecular mass of approximately 18.6 kDa. The protein migrates at about 35 kDa in DTT-reducing conditions by SDS-PAGE. The N-terminal amino acid is His.
- 途径
- Cancer Immune Checkpoints
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