Quick Overview for CD70 Protein (AA 39-193) (ABIN2666755)
抗原
See all CD70 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 39-193
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过滤
- 0.22 μm filtered
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 136,947.45 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 152,621.24 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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CD70 Molecule (CD70) (AA 39-193) protein (Fc Tag)
CD70 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD70 Molecule (CD70) protein (mFc-His Tag)CD70 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD70 Molecule (CD70) (AA 45-195) protein (Fc Tag)CD70 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD70 Molecule (CD70) (AA 50-193), (Trimer) protein (His tag)CD70 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD70 Molecule (CD70) (AA 50-193), (Trimer) protein (His tag,Biotin)CD70 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD70 Molecule (CD70) (AA 1-195) protein (His tag)custom-made CD70 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CD70 Molecule (CD70) (AA 1-193) protein (His tag)custom-made CD70 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CD70 Molecule (CD70) (AA 39-194) protein (His tag)CD70 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,
CD70 Molecule (CD70) (AA 39-193) protein (Fc-His Tag)CD70 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CD70 Molecule (CD70) (AA 52-193) protein (His-DYKDDDDK-Strep II Tag)CD70 宿主: 人 宿主: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Human CD27L induces proliferation of PHA-activated human T cells in a dose dependent manner. The ED50 is less than 1.0 μg/mL. Results may vary depending on human donor and dose of PHA.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- CD70 (CD70 Molecule (CD70))
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别名
- CD27L
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背景
- CD27L is a 50 kD type II transmembrane glycoprotein and member of the tumor necrosis factor superfamily. Human CD27L and its mouse counterpart share about 56 % sequence homology. The interaction of CD27L and CD27, a protein of the TNF receptor superfamily expressed on NK cells and subsets of T and B cells, provides co-stimulatory signals that are required for T cell proliferation, clonal expansion and development of effector T cells. CD27L-CD27 interaction on mouse B cells, on the other hand, has been shown to inhibit terminal differentiation of B cells into plasma cells. CD27L expression in mice is induced by B cell receptor activation in B cells, and at low levels by T cell receptor activation in T cells. CD27 interaction with recombinant soluble CD27L protein (sCD27L), showed that sCD27L enhanced IFN-γ secretion by CD56 (bright) NK cells in the presence of IL-12, and these NK cells were able to suppress HCV replication. Also, CD8+ T cells stimulated with sCD27L in the presence of antigen (Ag) showed enhanced proliferation and production of IL-2 and IFN-γ in vitro. In addition, administration of Ag and sCD27L induced expansion of Ag-specific CD8+ T cells in vivo. Data suggestes that sCD27L is a potent stimulator of antiglioma immune responses that depend on CD8+ T cells, therefore, sCD27L might be a good candidate as adjuvant in immunotherapy trials for glioblastoma.
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分子量
- The 176 amino acid recombinant protein has a predicted molecular mass of approximately 19.3 kDa. The protein migrates at about 35 kDa in DTT-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is His.
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途径
- Cancer Immune Checkpoints
抗原
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