IL36A/IL1F6 Protein (AA 6-158)
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Quick Overview for IL36A/IL1F6 Protein (AA 6-158) (ABIN2666739)
抗原
See all IL36A/IL1F6 (IL1F6) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 6-158
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Interleukin 1 Family, Member 6 (IL1F6) (Active) protein
IL1F6 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE. Active
Interleukin 1 Family, Member 6 (IL1F6) (AA 1-160) protein (His-IF2DI Tag)IL1F6 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Interleukin 1 Family, Member 6 (IL1F6) (AA 1-158) protein (His tag)IL1F6 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Interleukin 1 Family, Member 6 (IL1F6) (AA 1-158) protein (GST tag)IL1F6 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Interleukin 1 Family, Member 6 (IL1F6) (AA 8-160) (Active) proteinIL1F6 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by SDS-PAGE. ELISA, WB, SDS, AcA, Imm Active
Interleukin 1 Family, Member 6 (IL1F6) (AA 1-158) (Active) protein (His tag)IL1F6 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by SDS-PAGE. ELISA, WB, SDS, AcA, Imm Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: The ED50 is 20 - 50 ng/ml, corresponding to a specific activity 2 - 5 x 104 units/mg, as determined by a dose-dependent stimulation of IL-8 secretion in human preadipocytes.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA.
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缓冲液
- 0.22 μm filtered protein solution is in 20 mM Hepes pH 7.2, 150 mM NaCl, 10 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one week, at -20°C for three months, or at -70°C for 6 months.
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- IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))
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别名
- IL-36 alpha
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背景
- IL-36α is one of the IL-36 cytokines that are part of the IL-1 family. Like other IL-1 family members, IL-36α requires N-terminal processing to gain full bioactivity, currently, the proteases responsible for processing IL-36α are unknown. IL-36α signals through IL-36R/IL-1RAcP, which results in MAPK, Erk1/2, and JNK activation. IL-36α is implicated in skin homeostasis, and it is overexpressed in psoriatic lesional skin. Transgenic mice overexpressing IL-36α in skin have an inflammatory skin condition showing some characteristics of human psoriasis, including thickened scaly skin, acanthosis, hyperkeratosis, and dermis infiltration. EGF regulates the expression of IL-36α in the skin. IL-36α can also be detected in adipose tissue. IL-36α reduces adipocyte differentiation and also induces inflammatory gene expression in mature adipocytes. In lungs, the expression of IL-36α is increased in response to inflammatory stimuli. Intratracheal instillation of recombinant mouse IL-36α induces CXCL1 and CXCL2 expression and also neutrophil influx in the lungs. IL-36α, IL-36β, and IL-36γ induce in vitro expression of RNA of multiple cytokines (IL-6, IL-12 p40, CXCL1, CCL1, IL-12 p35, IL-1b, IL-19 p19, GM-CSF, CXCL10, TNFα, CCL3, VCAM-1, and ICAM-1) in mouse bone marrow-derived dendritic cells and CD4 T cells obtained from normal mice. IL-36α expression is elevated in chronic kidney disease and in rheumatoid arthritis synovium, and decreased expression correlates with a poor prognosis in hepatocellular carcinoma.
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分子量
- The 153 amino acid recombinant protein has a predicted molecular mass of approximately 17 kDa. The DTT-reduced and non-reduced protein migrate at approximately 17 kDa by SDS-PAGE. The predicted N-terminal amino acid is Lys.
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途径
- Cancer Immune Checkpoints
抗原
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