FGF21 Protein (AA 29-209, N-Term)
Quick Overview for FGF21 Protein (AA 29-209, N-Term) (ABIN2666736)
抗原
See all FGF21 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 29-209, N-Term
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Fibroblast Growth Factor 21 (FGF21) (AA 29-209) protein (mFc-Avi Tag)
FGF21 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Growth Factor 21 (FGF21) (AA 29-209) protein (His tag)FGF21 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Growth Factor 21 (FGF21) (AA 29-209) protein (mFc-Avi Tag,Biotin)FGF21 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Growth Factor 21 (FGF21) (AA 29-209) protein (His tag)FGF21 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Growth Factor 21 (FGF21) (AA 29-210) protein (His tag)FGF21 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Growth Factor 21 (FGF21) (Active) proteinFGF21 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Fibroblast Growth Factor 21 (FGF21) (AA 29-208) protein (His tag)FGF21 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibroblast Growth Factor 21 (FGF21) (AA 29-209) protein (Fc Tag)FGF21 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Fibroblast Growth Factor 21 (FGF21) (AA 30-209) protein (GST tag)FGF21 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: The ED50 is 0.05 - 0.4 μg/ml, corresponding to a specific activity 0.25 - 2 x 104 units/mg, as determined by a dose-dependent stimulation of NIH3T3 cell proliferation in the presence of 4 μg/ml of recombinant mouse β-klotho and 10 μg/ml heparin.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one week, at -20°C for three months, or at -70°C for 6 months.
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- FGF21 (Fibroblast Growth Factor 21 (FGF21))
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别名
- FGF-21
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背景
- FGF-21 is an atypical member of the FGF superfamily and functions as an endocrine hormone. It is mainly secreted by the liver but also by other tissues including adipocytes, pancreas, and skeletal muscle. It can act not only locally but also can travel through the circulatory system and function as an endocrine hormone. FGF-21 signals through a complex formed by classic FGF receptors and the coreceptor β-Klotho. Although it has been shown that FGF-21 can bind all FGFR isotypes, in vitro studies have shown that FGF-21 prefers FGFR1c/β-Klotho complex. Since the expression of β-Klotho is restricted to several tissues including liver, adipose tissues, pancreas, CNS and testes, it is believed that these tissues are the major sites of FGF-21 action. In liver, FGF-21 can induce gluconeogenesis, fatty acid oxidation, triglyceride degradation and ketogenesis. It has also been reported that PGC-1α, a key regulator of mitochondrial energy metabolism, can be induced by FGF-21. In adipose tissue, FGF-21 regulates lipolysis and stimulates glucose uptake independent of insulin. In pancreatic islets, FGF-21 can increase insulin expression. Transgenic mice overexpressing FGF-21 are protected from diet-induced obesity, therefore, FGF-21 has been considered a promising potential therapy for the treatment of obesity-related disorders. FGF-21 can cross the blood-brain barrier and FGF-21 has been shown to regulate peripheral metabolism and circadian behavior by acting on the nervous system.
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分子量
- The 187 amino acid recombinant protein has a predicted molecular mass of approximately 20 kDa. The DTT-reduced and non-reduced protein migrate at approximately 20 kDa by SDS-PAGE. The predicted N-terminal amino acid is Met.
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途径
- RTK signaling
抗原
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