FIL1h Protein (AA 31-183)
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北京 101111
Quick Overview for FIL1h Protein (AA 31-183) (ABIN2666729)
抗原
蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 31-183
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Interleukin 1 eta (FIL1h) (Active) protein
FIL1h 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 1 eta (FIL1h) (AA 1-157) protein (GST tag)FIL1h 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 1 eta (FIL1h) proteinFIL1h 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Interleukin 1 eta (FIL1h) (AA 31-183) protein (His-IF2DI Tag)FIL1h 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 1 eta (FIL1h) (AA 5-157) protein (His-IF2DI Tag)FIL1h 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: The ED50 is 0.1 - 0.5 ng/ml, corresponding to a specific activity 0.2 - 1.0 x 107 units/mg, as determined by a dose-dependent stimulation of IL-6 secretion in 3T3L1 preadipocytes.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA.
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缓冲液
- 0.22 μm filtered protein solution is in 20 mM Tris pH 7.0, 150 mM, 2 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one week, at -20°C for six months, or at -70°C for one year.
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- FIL1h (Interleukin 1 eta (FIL1h))
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别名
- IL-36 beta
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背景
- The IL-1 family is a group of cytokines comprised of 11 members, including the IL-36 cytokines, IL-36α, β and γ (previously known as IL-1F6, IL-1F8, and IL-1F9). Like other IL-36 cytokines, IL-36β signals through IL-1Rrp2 and IL-1RAcP and activate NF-κB and MAPKs. IL-36β functions as a chemokine and induces migration of bone marrow-derived dendritic cells (BMDCs) and CD4+ T cells. IL-36β induces proinflammatory cytokines, including IL-12, IL-1β, IL-6, and TNF-α in BMDCs. Furthermore, it has been shown that IL-36β synergizes with IL-12 to enhance Th1 polarization. IL-36β is also expressed in neurons and glia cells, but recombinant IL-36β does not induce the classical IL-1 response in brain cells. In a cultured keratinocyte system, IL-36β can induce robust expression of many chemokines in macrophages, T cells, and neutrophils. The secretion of antimicrobial peptides, including HBD-2, HBD-3, lipocalin 2, CAMP, elafin, serpinB1, and IL-8 can be induced by IL-36β in cultured keratinocytes. IL-36β, can also upregulate MMP9 and MMP19 mRNA in cultured keratinocytes. IL-36β has been implicated in the development of human psoriasis. IL-36β is expressed in the skin and the expression can be upregulated by IL-1β, TNF-α, flagellin, and poly (I:C). The expression of IL-36β is also increased in plaque psoriasis. In addition, IL-36β can be detected in mononuclear cells that infiltrate lesional skin. In mouse models of psoriasis, IL-36β is significantly upregulated in inflamed skin.
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分子量
- The 153 amino acid recombinant protein has a predicted molecular mass of approximately 17 kDa. The DTT-reduced and non-reduced protein migrates at approximately 17 kDa by SDS-PAGE. The predicted N-terminal amino acid is Ser.
抗原
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