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Quick Overview for FIL1h Protein (AA 31-183) (ABIN7320596)
抗原
蛋白类型
宿主
资源
纯度
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产品特性
- AA 31-183
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原理
- Recombinant Mouse Interleukin-36b/IL-36b Protein
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序列
- Ser31-Lys183
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产品特性
- Recombinant Mouse Interleukin-36 beta is produced by our E.coli expression system and the target gene encoding Ser31-Lys183 is expressed.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Interleukin 1 eta (FIL1h) (Active) protein
FIL1h 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 1 eta (FIL1h) (AA 1-157) protein (GST tag)FIL1h 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 1 eta (FIL1h) (AA 31-183) protein (His-IF2DI Tag)FIL1h 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interleukin 1 eta (FIL1h) (AA 5-157) protein (His-IF2DI Tag)FIL1h 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM Nacl, 1 mM EDTA, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- FIL1h (Interleukin 1 eta (FIL1h))
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别名
- Interleukin-36b/IL-36b
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背景
- Il1f,Interleukin-1 family member,IL-1F,IL-1F8,Interleukin-1 family member 8,Interleukin-36 beta,Fil1e,Il1f8,Il36b,Interleukin 36 beta (IL-36B) is a member of the IL-1 family of proteins. It is a cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. IL-36B is synthesized in several cells including resting and activated monocytes, and B cells. The receptor for IL-36 beta is thought to be a combination of IL-1 Rrp2 and IL-1 RAcP. Interleukin 36 beta is one part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response, similar to the IL-1 system with which it shares the coreceptor IL1RAP. IL36B are involved in a number of fundamental biological processes such as stimulating production of interleukin-6 and interleukin-8, inducing expression of a number of antimicrobial peptides including beta-defensin 4 and beta-defensin 103 as well as a number of matrix metalloproteases , inducing the production of proinflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), and activating p38 MAPK phosphorylation in BMDCs. Moreover, interleukin 36 beta may be involved in skin inflammatory response by acting on keratinocytes, dendritic cells, and indirectly on T cells to drive tissue infiltration, cell maturation and cell proliferation. It plays an important role in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II and inducing the production of IFN-gamma, IL-4 and IL-17 by T helper 1 (Th1) cells, cultured CD4+ T cells and splenocytes.
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分子量
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Calculated MW: 17.6 kDa
Observed MW: 16 kDa
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UniProt
- Q9D6Z6
抗原
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