IL-15 Protein (AA 49-162)
Quick Overview for IL-15 Protein (AA 49-162) (ABIN2666688)
抗原
See all IL-15 (IL15) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 49-162
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内毒素水平
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Less than 1 EU/μg cytokine as determined by the LAL method.
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Interleukin 15 (IL15) (AA 49-162) protein
IL15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by Tris-Bis PAGE, > 95 % as determined by SEC-HPLC
Interleukin 15 (IL15) (AA 50-162) (Active) protein (His tag)IL15 宿主: 人 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
Interleukin 15 (IL15) (AA 49-162) protein (His-IF2DI Tag)IL15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 15 (IL15) (AA 1-162) protein (GST tag)IL15 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 15 (IL15) (AA 49-162), (full length) protein (His tag)IL15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag)IL15 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (His tag)IL15 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) proteinIL15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag,AVI tag,Biotin)IL15 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag,AVI tag,Biotin)IL15 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50=2.5 - 15 ng/ml, corresponding to a specific activity of 0.6 - 4 x 105 units/mg, as determined by the dose dependent stimulation of CTLL-2 cell proliferation.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- Lyophilized from a concentrated solution (1 mg/mL) with 10 mM NaH2PO4, 150 mM NaCL, pH 8.0.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- IL-15 (IL15) (Interleukin 15 (IL15))
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别名
- IL-15
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背景
- IL-15 was discovered in the supernatant of Simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2. IL-15 is a regulatory cytokine, and it is produced by dendritic cells, epithelial cells, fibroblasts, and monocytes. IL-15 plays an important role in immune response and shares many functions with IL-2. For example, it stimulates the proliferation of activated T cells, NK cells, and B cells, and induces immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells and induces the production of proinflammatory cytokines from macrophages. IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. IL-15 shares the receptor chains β and γc with IL-2. IL-15 is normally not secreted in soluble form but is instead held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 is then presented in trans form to T cells and NK cells and is recognized by the γc receptor on these cells, such recognition maintains cell survival and intermittent proliferation.
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分子量
- The 115 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 13,382 Da.
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途径
- JAK/STAT Signaling, Glycosaminoglycan Metabolic Process
抗原
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