IL-34 Protein (AA 1-235)
This Recombinant IL-34 protein is expressed in CHO Cells.
Quick Overview for IL-34 Protein (AA 1-235) (ABIN2666664)
抗原
See all IL-34 (IL34) 蛋白
IL-34 (IL34)
(Interleukin 34 (IL34))
蛋白类型
Recombinant
生物活性
Active
宿主
小鼠
资源
CHO Cells
应用范围
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)
纯度
> 98 % , as determined by Coomassie stained SDS-PAGE.
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产品特性
- AA 1-235
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: ED50 =10 - 50 ng/ml, corresponding to a specific activity of 0.2 - 1 x 105 units/mg, as determined by M-NFS-60 cell proliferation induction in a dose dependent manner.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 0.5 M NaCl, pH 7.4
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- IL-34 (IL34) (Interleukin 34 (IL34))
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别名
- IL-34
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背景
- Mouse IL-34 shares a sequence identity of 71 % with human IL-34 on the amino acid level, and the IL-34 gene is syntenic in the human, chimpanzee, rat, and mouse genomes. IL-34 has no apparent consensus structural domain or motif and does not share sequence homology with M-CSF, nevertheless, it binds to the CSFR. These two cytokines are not identical in biological activity and signal activation. IL-34 and CSF show an equivalent ability to support cell growth or survival. However, these cytokines have different ability to induce the production of chemokines (MCP-1 and eotaxin-2) in primary macrophages, the morphological change in TF-1-fms cells, and the migration of J774A.1 cells. The use of monoclonal antibodies against the CSFR suggests a differential domain binding in the receptor to IL-34 and CSF. As a result, different bioactivities and signal activation kinetics/strength are produced for these cytokines.
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分子量
- The 215 amino acid recombinant protein has a predicted molecular mass of 26 kDa. The DTT-reduced protein migrates at approximately 39 kDa and the non-reduced protein with slightly greater mobility by SDS-PAGE.
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途径
- RTK signaling
抗原
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