IL-21 Protein (AA 30-162)
Quick Overview for IL-21 Protein (AA 30-162) (ABIN2666654)
抗原
See all IL-21 (IL21) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 30-162
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Interleukin 21 (IL21) (AA 32-162) protein
IL21 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 21 (IL21) (AA 30-162) protein (Fc Tag)IL21 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Interleukin 21 (IL21) (AA 30-162) (Active) proteinIL21 宿主: 人 宿主: HEK-293 Cells Recombinant >98 % as determined by SDS-PAGE. Active
Interleukin 21 (IL21) (AA 32-162) protein (His-Avi Tag,Biotin)IL21 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 21 (IL21) (AA 18-146) protein (His-IF2DI Tag)IL21 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 21 (IL21) (AA 25-155) protein (His-IF2DI Tag)IL21 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 21 (IL21) proteinIL21 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Interleukin 21 (IL21) protein (His tag)IL21 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Interleukin 21 (IL21) (AA 18-146) protein (His-Avi Tag,Biotin)IL21 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 21 (IL21) (AA 1-162) protein (GST tag)IL21 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: The ED50 is 0.3-0.5 ng/ml, corresponding to a specific activity 2.0-3.3 x 106 units/mg as determined by a dose-dependent induction of IFNγ secretion by NK-92 cells.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- IL-21 (IL21) (Interleukin 21 (IL21))
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别名
- IL-21
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背景
- IL-21 was identified by a functional screening from conditioned medium of activated T cells using Baf3-IL-21R transfectants. In normal B cells, IL-21 can mediate cell proliferation, growth arrest, and terminal differentiation, or apoptosis, depending on their activation status. IL-21 enhances B cell proliferation following incubation with an activating CD40 antibody. This result suggests that IL-21 enhances B cell function following T:B cell interactions. Nevertheless, IL-21 inhibits the proliferation of murine and human B cells stimulated with anti-IgM antibodies. In addition, IL-21 enhances proliferation, cytotoxic activity, and IFNγ production by CD8-effector T cells, and induces terminal differentiation of activated natural killer (NK) cells. Also, IL-21 can drive Th17 responses in conjunction with TGF-β. Nevertheless, IL-21 is not essential for the differentiation of Th17 cells in vitro or in vivo, as it was showed using IL-21 and IL-21R-deficient mice. IL-6 induces the production of IL-21 from CD4 T cells upon TCR stimulation. In addition, IL-6 and IL-21 are key players in the Tfh differentiation, characterized by increased protein expression of both Bcl-6 and CXCR5. IL-21R complex is formed with the IL-21R-alpha chain and the common subunits (γc) shared with other interleukins, such as IL-2, IL-4, IL-7, IL-9, and IL-15.
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分子量
- The 133 amino acid recombinant protein has a predicted molecular mass of approximately 15.5 kDa. The DTT-reduced and non-reduced protein migrate at approximately 16 kDa by SDS-PAGE. The N-terminal amino acid is Glutamine.
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原
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