CCL20 Protein (AA 27-96)
Quick Overview for CCL20 Protein (AA 27-96) (ABIN2666608)
抗原
See all CCL20 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 27-96
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过滤
- 0.22 μm filtered
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内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Chemokine (C-C Motif) Ligand 20 (CCL20) protein (His tag)
CCL20 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Chemokine (C-C Motif) Ligand 20 (CCL20) (AA 27-95) protein (GST tag)CCL20 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Bioactivity was measured by its property to chemoattract Baf3-hCCR6 transfectants in a dose dependent manner.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
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缓冲液
- 0.22 μm filtered protein solution is in PBS.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
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别名
- CCL20
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背景
- CCL20 is an inflammatory cytokine involved in the recruitment of leukocytes to sites of injury and inflammation. CCL20 is expressed at a low basal level, but it is induced by proinflammatory cytokines and Toll-like receptor (TLR) agonists. During infections, CCL20 expression is stimulated in epithelial cells, and immature CCR6+ dendritic cells (DCs) are recruited. Thereafter, CCR6 is downregulated during DC maturation, and the DCs migrates to the lymph node under the expression of CCR7. CCL20 is the only ligand for CCR6. Nevertheless, mouse and human β -defensin-1 and -2, small cationic antimicrobial peptides, bind and activate CCR6. Contrarily, these β-defensins have lower affinities for CCR6 than CCL20. In fact, human CCL20 has potent antimicrobial activity.
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分子量
- The 70 amino acid recombinant protein has a predicted molecular mass of approximately 8.0 kDa. The DTT-reduced protein migrates at approximately 11 kDa and non-reduced protein at approximately 12 kDa by SDS-PAGE. The N-terminal amino acid is Ala.
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途径
- The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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