FGF3 Protein (AA 28-212, N-Term)
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- 抗原 See all FGF3 蛋白
- FGF3 (Fibroblast Growth Factor 3 (FGF3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 28-212, N-Term
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.01ng per μg cytokine as determined by the LAL method.
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- Discover our top product FGF3 蛋白
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Fibroblast Growth Factor 3 (FGF3) (Active) protein
FGF3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Fibroblast Growth Factor 3 (FGF3) (AA 13-239) protein (His tag)FGF3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: The ED50 is 100 - 200 ng/ml, corresponding to a specific activity of 5.0 -10 x 103 units/mg, as determined by a dose-dependent stimulation of NIH3T3 cell proliferation in the presence of 2 μg/ml of heparin.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
- 缓冲液
- 0.22 μm filtered protein solution is in 20 mM Tris, pH 7.0, 100 mM NaCl, 1 mM EDTA, and 10 % Glycerol.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Human FGF-3 is unstable at room temperature. Unopened vial can be stored at -20°C for six months, or at -70°C for one year.
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- 抗原
- FGF3 (Fibroblast Growth Factor 3 (FGF3))
- 别名
- FGF-3 (FGF3 产品)
- 别名
- HBGF-3 Protein, INT2 Protein, Int2 Protein, Fgf-3 Protein, Int-2 Protein, Int-P Protein, INT-2 Protein, XFGF-3 Protein, fgf3-A Protein, xfgf3 Protein, FGF-3 Protein, id:ibd5006 Protein, fibroblast growth factor 3 Protein, fibroblast growth factor 3 L homeolog Protein, FGF3 Protein, Fgf3 Protein, fgf3.L Protein, fgf3 Protein
- 背景
- FGF-3 is a member of the fibroblast growth factor family. The mouse FGF-3, originally named Int-2, was identified as a proto-oncogene that is activated by nearby integration of mouse mammary tumor viruses in virus-induced tumors. The amplification of the FGF-3 gene has been found in many cancer types including squamous cell carcinoma, epithelial ovarian tumors, breast cancer, and bladder cancer. FGF-3 is also associated with tumor metastasis and recurrence in human hepatocellular carcinoma. Like many other FGF proteins, FGF-3 plays important roles during embryonic development. FGF-3 is required for normal inner ear development including placode induction, maintenance, and otic vesicle formation. The role of FGF-3 in ear development is conserved amongst different vetebrates including mouse, chicken, and zebrafish. It has been shown that FGF-3, FGF-8, and FGF-10 play redundant and unique roles in ear development. FGF-3 is also important for early hindbrain patterning. The hindbrain boundary cells-derived FGF-3 regulates the expression of multiple markers at hindbrain boundaries. The involvement of FGF-3 in cardiovascular development also has been reported and is described to be in two forms. The two forms of FGF-3 describe a secreted form that induces proliferation and another form that localizes in the nucleus and inhibits cell proliferation. The nuclear isoform binds rpS2 and this binding has been suggested to interfere with ribosomal biogenesis.
- 分子量
- The 192 amino acid recombinant protein has a predicted molecular mass of approximately 22 kDa. The DTT-reduced and non-reduced protein migrate at approximately 22 kDa by SDS-PAGE. The predicted N-terminal amino acid is Met.
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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