FGF2 蛋白
(1 reference)Quick Overview for FGF2 蛋白 (ABIN2129216)
抗原
See all FGF2 蛋白蛋白类型
生物活性
宿主
资源
纯度
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序列
- MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
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产品特性
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Purified recombinant Human FGF basic protein
Expression System: E.coli
Bioactivity: The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is
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Fibroblast Growth Factor 2 (Basic) (FGF2) (full length) (Active) protein
FGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE CC Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 135-288), (Isoform 1) (Active) proteinFGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE analysis. Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) (Active) protein (AVI tag,His tag,Biotin)FGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by SDS-PAGE. Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (Active) protein (His tag)FGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 9-154) (Active) protein (His tag)FGF2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 189-288) protein (GST tag)FGF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Fibroblast Growth Factor 2 (Basic) (FGF2) proteinFGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
Fibroblast Growth Factor 2 (Basic) (FGF2) proteinFGF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Fibroblast Growth Factor 2 (Basic) (FGF2) proteinFGF2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Fibroblast Growth Factor 2 (Basic) (FGF2) proteinFGF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
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应用备注
- Each Investigator should determine their own optimal working dilution for specific applications.
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说明
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Biological activity: The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/mL, corresponding to a specific activity of 2,000,000 Units/mg.
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实验流程
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Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in distilled water to at least 100 μg/mL.
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缓冲液
- Supplied in lyophilized form containing 10 mM Acetic acid,
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- RT/-20 °C
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: "Enhancing inflammatory and chemotactic signals to regulate bone regeneration." in: European cells & materials, Vol. 28, pp. 320-34, (2014) (PubMed).
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: "Enhancing inflammatory and chemotactic signals to regulate bone regeneration." in: European cells & materials, Vol. 28, pp. 320-34, (2014) (PubMed).
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- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
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别名
- FGF Basic
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背景
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FGF basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF basic signals through FGFR 1b, 1c, 2c, 3c and 4.
Alternative Names: FGF-2 protein, HBGH-2 protein, HBGF-2 protein, FGF-b protein, Fibroblast Growth Factor Basic protein, Prostatropin protein -
分子量
- 17,353 Da
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins
抗原
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