Quick Overview for IL-4 Protein (AA 25-153) (ABIN2018117)
抗原
See all IL-4 (IL4) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 25-153
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产品特性
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ED50< 0.25 ng/mL, measured in a cell proliferation assay using TF-1 human erythroleukemic cells, corresponding to a specific activity of >4 x 10^6 units/mg
AA 25-153, expressed with an N-terminal Met. -
内毒素水平
- < 0.2 EU/μg, determined by LAL method.
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Interleukin 4 (IL4) (AA 25-153) protein
IL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 4 (IL4) (AA 25-153) protein (His tag)IL4 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 4 (IL4) (AA 25-153) protein (His-Avi Tag,Biotin)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 4 (IL4) (AA 25-153) protein (His-Avi Tag)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 4 (IL4) (AA 23-140) (Active) proteinIL4 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag,AVI tag,Biotin)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 4 (IL4) protein (His tag)IL4 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 4 (IL4) (AA 25-153) (Active) proteinIL4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95% by SDS-PAGE SDS, AcA Active
Interleukin 4 (IL4) (AA 63-153) protein (GST tag)IL4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 4 (IL4) (AA 25-153) protein (mFc Tag)IL4 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstituted in ddH2O or PBS at 100 μg/mL.
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缓冲液
- Lyophilized after extensive dialysis against PBS.
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储存条件
- -80 °C
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储存方法
- Lyophilized recombinant human Interleukin 4 (IL-4) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhIL-4 should be stable up to 1 week at 4 °C or up to 2 months at -20 °C
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有效期
- 6 months
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- IL-4 (IL4) (Interleukin 4 (IL4))
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别名
- Interleukin-4 (IL-4)
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背景
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Interleukin-4 (IL-4) is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It has important effects on the growth and differentiation of different immunologically competent cells. Interleukin-4 is produced by mast cells, T cells, and bone marrow stromal cells[1]. IL-4 regulates the differentiation of native CD4+ T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 has numerous important biological functions including stimulating B-cells activation, T-cell proliferation and CD4+ T-cells differentiation to Th2 cells. It is a key regulator in hormone control and adaptive immunity[2]. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells[3]. IL-4 is stabilized by three disulphide bonds forming a compact globular protein structure[4]. Four alpha-helix bundle with left-handed twist is dominated half of the protein structure with 2 overhand connections and fall into a 2-stranded anti-parallel beta sheet[5].Recombinant human IL-4 (rhIL-4) is a 129 amino acid protein expressed in mammalian CHO system. The approximate molecular weight is 15 kDa analyzed by non-reducing SDS-PAGE.
Synonyms: IL-4, B cell growth factor-1 (BCGF-1), B cell stimulatory factor-1 (BSF-1), B cell differentiation factor (BCDF), T cell growth factor-2 (TCGF-2), Mast-cell growth factor-2 (MCGF-2) -
分子量
- 15 kDa, observed by non-reducing SDS-PAGE.
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NCBI登录号
- NP_000580
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
抗原
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