EIF4E Protein (AA 1-217)
Quick Overview for EIF4E Protein (AA 1-217) (ABIN1945364)
抗原
See all EIF4E 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 1-217
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原理
- Recombinant Human Eukaryotic Translation Initiation Factor 4E/EIF4E
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序列
- MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV
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产品特性
- Recombinant Human eIF-4E is produced by our E. coli expression system. The target protein is expressed with sequence (AA 1-217) of Human eIF-4E .
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 1-217) protein (GST tag)
EIF4E 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 1-217) protein (His tag)EIF4E 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Eukaryotic Translation Initiation Factor 4E (EIF4E) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)EIF4E 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
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注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
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别名
- Eukaryotic Translation Initiation Factor 4E
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背景
- Eukaryotic translation initiation factor 4E is a 217 amino acids protein that belongs to the eukaryotic initiation factor 4E family. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners.It can recognize and bind the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
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分子量
- 25.1 kDa
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UniProt
- P06730
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途径
- BCR Signaling
抗原
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