ATG5 Protein (AA 1-275)
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- 抗原 See all ATG5 蛋白
- ATG5 (ATG5 Autophagy Related 5 (ATG5))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-275
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 原理
- Recombinant Human Autophagy Related 5 Homolog/ATG5
- 序列
- MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLP RVSYLTLVTD KVKKHFQKVM RQEDISEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNI TVHFKSFPEK DLLHCPSKDA IEAHFMSCMK EADALKHKSQ VINEMQKKDH KQLWMGLQND RFDQFWAINR KLMEYPAEEN GFRYIPFRIY QTTTERPFIQ KLFRPVAADG QLHTLGDLLK EVCPSAIDPE DGEKKNQVMI HGIEPMLETP LQWLSEHLSY PDNFLHISII PQPTD
- 产品特性
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Recombinant Human Autophagy Related 5 Homolog/ATG5
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product ATG5 蛋白
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ATG5 Autophagy Related 5 (ATG5) (AA 138-275) protein (His-IF2DI Tag)
ATG5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
ATG5 Autophagy Related 5 (ATG5) (AA 1-275) protein (GST tag)ATG5 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
ATG5 Autophagy Related 5 (ATG5) (AA 176-275) protein (GST tag)ATG5 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
ATG5 Autophagy Related 5 (ATG5) (AA 1-275) protein (Strep Tag)Crystallography grade ATG5 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
ATG5 Autophagy Related 5 (ATG5) protein (Myc-DYKDDDDK Tag)ATG5 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM Tris,0.2M NaCl,1 mM DTT,40 % glycerol, pH 8.0 .
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- ATG5 (ATG5 Autophagy Related 5 (ATG5))
- 别名
- Autophagy Protein 5/ATG5 (ATG5 产品)
- 别名
- APG5 Protein, APG5-LIKE Protein, APG5L Protein, ASP Protein, hAPG5 Protein, ATG5 Protein, CG1643 Protein, DmAtg5 Protein, Dmel\\CG1643 Protein, atg5 Protein, 2010107M05Rik Protein, 3110067M24Rik Protein, AW319544 Protein, Apg5l Protein, Atg5l Protein, C88337 Protein, Paddy Protein, apg5l Protein, zgc:100934 Protein, ATATG5 Protein, AUTOPHAGY 5 Protein, MKP11.20 Protein, MKP11_20 Protein, autophagy related 5 Protein, Autophagy-related 5 Protein, ATG5 autophagy related 5 homolog (S. cerevisiae) Protein, autophagy related 5 L homeolog Protein, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 Protein, autophagy protein Apg5 family Protein, ATG5 Protein, Atg5 Protein, atg5 Protein, atg5.L Protein, APG5 Protein
- 背景
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Recombinant Human Autophagy protein 5/ATG5 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asp275) of Human ATG5.
ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton. - 分子量
- 32.4 kDa
- UniProt
- Q9H1Y0
- 途径
- Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy
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