UBE2M Protein (AA 1-183)
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- 抗原 See all UBE2M (ube2m) 蛋白
- UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-183
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 原理
- Recombinant Human NEDD8-Conjugating Enzyme UBC12/UBE2M
- 序列
- MIKLFSLKQQ KKEEESAGGT KGSSKKASAA QLRIQKDINE LNLPKTCDIS FSDPDDLLNF KLVICPDEGF YKSGKFVFSF KVGQGYPHDP PKVKCETMVY HPNIDLEGNV CLNILREDWK PVLTINSIIY GLQYLFLEPN PEDPLNKEAA EVLQNNRRLF EQNVQRSMRG GYIGSTYFER CLK
- 产品特性
- Recombinant Human NEDD8-conjugating enzyme Ubc12/UBE2M is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys183) of Human UBE2M.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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- Discover our top product ube2m 蛋白
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Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 1-183) protein (His tag)
custom-made ube2m 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin-Conjugating Enzyme E2M (ube2m) protein (His tag)ube2m 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 1-183) protein (GST tag)ube2m 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of 50 mM HEPES, pH 7.5,2 mM DTT,150 mM NaCl,10 % glycerol .
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
- 别名
- NEDD8-Conjugating Enzyme Ubc12/UBE2M (ube2m 产品)
- 背景
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UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. UBE2M accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. It involved in cell proliferation and is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation.
Alternative Names: NEDD8-conjugating enzyme Ubc12, NEDD8 carrier protein, NEDD8 protein ligase, Ubiquitin-conjugating enzyme E2 M, UBC12, UBE2M, - 分子量
- 20.9 kDa
- UniProt
- P61081
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