VEGFA Protein (Homodimer)
Quick Overview for VEGFA Protein (Homodimer) (ABIN1589664)
抗原
See all VEGFA 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- Homodimer
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原理
- VEGF164
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序列
- APTTEGEQKS HEVIKFMDVY QRSYCRPIET LVDIFQEYPD EIEYIFKPSC VPLMRCAGCC NDEALECVPT SESNITMQIM RIKPHQSQHI GEMSFLQHSR CECRPKKDRT KPENHCEPCS ERRKHLFVQD PQTCKCSCKN TDSRCKARQL ELNERTCRCD KPRR
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特异性
- Chromosomal location:17C, 17 24.2cM
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产品特性
- Length (aa):164
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Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (His tag)
VEGFA 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (Fc Tag)VEGFA 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) (Active) protein (His tag,AVI tag,Biotin)VEGFA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-146) (Active) protein (His tag,AVI tag,Biotin)VEGFA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Vascular Endothelial Growth Factor A (VEGFA) proteinVEGFA 宿主: 大鼠 宿主: 酵母菌 Recombinant > 95 % as determined by SDS-PAGE
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (His tag)VEGFA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Vascular Endothelial Growth Factor A (VEGFA) proteinVEGFA 宿主: 小鼠 宿主: Yeast (Pichia pastoris) Recombinant > 95 % as determined by SDS-PAGE
Vascular Endothelial Growth Factor A (VEGFA) (AA 1-147) protein (GST tag)VEGFA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-146) protein (His tag)VEGFA 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS
Vascular Endothelial Growth Factor A (VEGFA) proteinVEGFA 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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应用备注
- The ED50 for stimulation of cell proliferation by human umbilical vein endothelial cells for VEGF164 has been determined to be in the range of 1-5 ng/mL.
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说明
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Cytokines & Growth Factors
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- The lyophilized VEGF164 should be reconstituted in 50 mM acetic acid to a concentration not lower than 50 μg/mL. For long term storage we recommend to add at least 0.1 % human or bovine serum albumin.
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缓冲液
- 50 mM acetic acid
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C,-80 °C
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储存方法
- Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF164 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!
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有效期
- 6 months
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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别名
- VEGF164
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背景
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Mouse Vascular Endothelial Growth Factor164 (VEGF164), a 24 kDa protein consisting of 164 amino acid residues, is produced as a homodimer. VEGF164 is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF164 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (Flk-1). Consistent with the endothelial cell-specific action of VEGF164, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extravillous trophoblasts. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo and is also a chemoattractant for monocytes and endothelial cells. At least three different proteins are generated by differential splicing of the mouse VEGF gene: VEGF120, VEGF164 and VEGF188. The most abundant form is VEGF164. Whereas VEGF120 and VEGF164 are secreted proteins, VEGF188 is strongly cell-associated. In addition, the isoforms VEGF164 and VEGF188 bind to heparin with high affinity. VEGF is apparently a homodimer, but preparations of VEGF show some heterogenity on SDS gels depending of the secretion of different forms and the varying degrees of glycosylation. All dimeric forms possess similar biological activities. There is evidence that heterodimeric molecules between the different isoforms exist and that different cells and tissues express different VEGF isoforms. A related protein of VEGF is placenta growth factor (PlGF) with about 53% homology and VEGF-B with similar biological activities.
Synonyms: vascular endothelial growth factor A, Vegfa,Vpf, Vegf, Vegf164 -
分子量
- 48.0 kDa
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基因ID
- 22339
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NCBI登录号
- NM_001025250, NP_001020421
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UniProt
- Q00731
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途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原
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