FLT1 Protein (glycosylated, Monomer, Soluble)
Quick Overview for FLT1 Protein (glycosylated, Monomer, Soluble) (ABIN1589607)
抗原
See all FLT1 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- glycosylated, Monomer, Soluble
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原理
- VEGFR-1/Flt-1 (D3), soluble
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序列
- SKLKDPELSL KGTQHIMQAG QTLHLQCRGE AAHKWSLPEM VSKESERLSI TKSACGRNGK QFCSTLTLNT AQANHTGFYS CKYLAVPTSK KKETESAIYI FISDTGRPFV EMYSEIPEII HMTEGRELVI PCRVTSPNIT VTLKKFPLDT LIPDGKRIIW DSRKGFIISN ATYKEIGLLT CEATVNGHLY KTNYLTHRQT NTIIDVQIST PRPVKLLRGH TLVLNCTATT PLNTRVQMTW SYPDEKNKRA SVRRRIDQSN SHANIFYSVL TIDKMQNKDK GLYTCRVRSG PSFKSVNTSV HIYDKAFITV KHRKQQVLET VAGKRSY
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特异性
- Chromosomal location:13q12
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产品特性
- Length (aa):327
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Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (AA 27-756) protein (His-Avi Tag)
FLT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (AA 27-756) protein (His-Avi Tag,Biotin)FLT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (AA 27-756) protein (His tag)FLT1 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (AA 27-756) protein (Fc Tag)FLT1 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (AA 23-759) (Active) protein (Fc Tag)FLT1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >85 % as determined by SDS-PAGE. Active
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (AA 27-756) (Active) protein (His tag,AVI tag,Biotin)FLT1 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (Active) protein (His tag)FLT1 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1) (AA 581-687) protein (GST tag)FLT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
- The activity of sVEGFR-1(D3) was determined by its ability to inhibit the VEGF-A-induced proliferation of HUVECs.
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说明
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Soluble Receptors
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- The lyophilized sVEGFR-1(D3) is soluble in water and most aqueous buffers and should be reconstituted in PBS to a concentration not lower than 100 μg/mL.
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缓冲液
- PBS
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储存条件
- -20 °C,-80 °C
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储存方法
- Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted sVEGFR-1(D3) should be stored in working aliquots at -70°C.
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有效期
- 6 months
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- FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))
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别名
- VEGFR-1/Flt-1
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背景
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Recombinant human soluble Vascular Endothelial Growth Factor Receptor-1 domain D1-3 (sVEGFR-1(D3)) is produced as a non-chimeric protein in a monomeric form. The soluble receptor protein contains only the first 3 extracellular domains, which contain all the information necessary for binding of VEGF. The receptor monomers have a mass of approximately 45 kDa containing 352 amino acid residues. Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis, binding VEGF with the same affinity as the full-length receptor.
Synonyms: soluble vascular endothelial growth factor receptor-1, soluble FLT1, soluble VEGFR-1 -
分子量
- 45.0 kDa
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基因ID
- 2321
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NCBI登录号
- NM_001159920, NP_001153392
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UniProt
- P17948
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途径
- RTK signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
抗原
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