PDGFC Protein (Homodimer)
This Recombinant PDGFC protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for PDGFC Protein (Homodimer) (ABIN1589585)
抗原
See all PDGFC 蛋白
PDGFC
(Platelet-Derived Growth Factor C (PDGFC))
蛋白类型
Recombinant
生物活性
Active
宿主
Parapoxvirus
资源
大肠杆菌（E. Coli）
纯度
> 90 % by SDS-PAGE
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产品特性
- Homodimer
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原理
- VEGF-E
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序列
- MGSSHHHHHH SSGLVPRGSH DSTKTWSEVF ENSGCKPRPM VFRVHDEHPE LTSQRFNPPC VTLMRCGGCC NDESLECVPT EEANVTMQLM GASVSGGNGM QHLSFVEHKK CDCKPPLTTT PPTTTRPPRR RR
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产品特性
- Length (aa):132
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内毒素水平
- < 0.1 ng per μg of ov-VEGF-E
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Platelet-Derived Growth Factor C (PDGFC) (AA 236-345) protein (GST tag)
PDGFC 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Platelet-Derived Growth Factor C (PDGFC) (AA 1-345) protein (GST tag)PDGFC 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
- Measured in a cell proliferation assay using primary HUVECs. The ED50 for this effect is typically 1 - 5 ng/mL.
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说明
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Cytokines & Growth Factors
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- The lyophilized ovVEGF-E should be reconstituted in water or medium to a concentration not lower than 50 μg/mL. For long term storage we would recommend to add at least 0.1 % human or bovine serum albumin.
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缓冲液
- PBS
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- PDGFC (Platelet-Derived Growth Factor C (PDGFC))
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别名
- VEGF-E
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物质类
- Viral Protein
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背景
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A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999, GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al., 1999, Wise et al., 1999). Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E can not bind to VEGF receptor-1 (Flt-1). Furthermore VEGF-E can also not bind to VEGF receptor-3 (FLT-4). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor –2/KDR.
Synonyms: Vascular Endothelial Growth Factor-E -
分子量
- 35 kDa (Dimer)
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UniProt
- Q9YMF3
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途径
- RTK signaling, Platelet-derived growth Factor Receptor Signaling
抗原
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