TNFSF13 Protein (AA 18-111, AA 88-232) (DYKDDDDK Tag)
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- 抗原 See all TNFSF13 蛋白
- TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 18-111, AA 88-232
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宿主
- 人, 小鼠
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资源
- HEK-293 Cells
- 标记
- This TNFSF13 protein is labelled with DYKDDDDK Tag.
- 应用范围
- SDS-PAGE (SDS)
- 原理
- APRIL (mouse) (multimeric) (rec.)
- 品牌
- MegaAPRIL™
- 特异性
- Binds to human and mouse BCMA and TACI. Binds to proteoglycans.
- 交叉反应
- 人, 小鼠
- 产品特性
- The extracellular domain of mouse APRIL (aa 88-232) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.
- 纯度
- >95 % (SDS-PAGE)
- 内毒素水平
- <0.02EU/μg purified protein (LAL test).
- Biological Activity Comment
- Stimulates B cell proliferation.
- Top Product
- Discover our top product TNFSF13 蛋白
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Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (Trimer) protein (His-DYKDDDDK Tag)
TNFSF13 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 96-241) protein (Fc Tag)TNFSF13 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (Trimer) protein (His-Avi-DYKDDDDK Tag)TNFSF13 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 1-247) protein (GST tag)TNFSF13 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 111-250) (Active) protein (His tag,DYKDDDDK Tag)TNFSF13 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 105-250) (Active) protein (Fc Tag,AVI tag,Biotin)TNFSF13 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (Trimer) protein (His-Avi-DYKDDDDK Tag,Biotin)TNFSF13 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) protein (Fc Tag)TNFSF13 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- 0.1 mg/mL
- 缓冲液
- Contains PBS + 0.5 % Trehalose.
- 注意事项
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
- 有效期
- 6 months
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: "Selective APRIL blockade delays systemic lupus erythematosus in mouse." in: PLoS ONE, Vol. 7, Issue 2, pp. e31837, (2012) (PubMed).
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: "Selective APRIL blockade delays systemic lupus erythematosus in mouse." in: PLoS ONE, Vol. 7, Issue 2, pp. e31837, (2012) (PubMed).
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- 抗原
- TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))
- 别名
- APRIL (TNFSF13 产品)
- 背景
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MultimericAPRIL™, ACRP30headless:APRIL, ACRP30headless:CD256, ACRP30headless:TNFSF13, ACRP30headless:A-proliferation-inducing Ligand:
APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. MultimericAPRIL™ is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the N-terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells.
- 分子量
- ~34kDa (SDS-PAGE)
- UniProt
- Q9D777
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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