IL-4 蛋白
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北京 101111
Quick Overview for IL-4 蛋白 (ABIN1305127)
抗原
See all IL-4 (IL4) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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特异性
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Biological Activity: Proliferation is measured using MC/9 indicator cells pulsed with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide).
Specific Activity: 0.3 - 2.0 x 10^8 Units/mg
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.
ED50: 50 - 300 pg/mL -
产品特性
- Recombinant mouse IL-4 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, pH 7.2, containing glycerol and 2.0 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant mouse IL-4 was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers Lambert Law.
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纯化方法
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Immunoaffinity Chromatography
Recombinant mouse IL-4 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives. Recombinant mouse IL-4 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. -
过滤
- 0.22 μm filtered
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内毒素水平
- The endotoxin level is ≤ 0.1 ng per µg of mouse IL-4, as measured in a chromogenic LAL assay.
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Interleukin 4 (IL4) (AA 25-153) protein (His tag)
IL4 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 4 (IL4) (AA 25-153) protein (His-Avi Tag,Biotin)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 4 (IL4) (AA 25-153) protein (His-Avi Tag)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 4 (IL4) (AA 23-140) (Active) proteinIL4 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 4 (IL4) (AA 25-153) (Active) proteinIL4 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag,AVI tag,Biotin)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 4 (IL4) protein (His tag)IL4 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag)IL4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Interleukin 4 (IL4) (AA 63-153) protein (GST tag)IL4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 4 (IL4) (AA 25-153) protein (mFc Tag)IL4 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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应用备注
- ELISA Standard: Mouse IL-4 is useful as a quantitative standard for measuring mouse IL-4 protein levels in an IL-4 specific sandwich ELISA with the purified 11B11 antibody (Cat. No. 554434) as a capture antibody and the biotinylated BVD6-24G2 (Cat. No. 554390) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse IL-4 standard from ~2,000 to 15 pg/mL should be included in each ELISA plate. For assay of mouse IL-4 in complex fluids such as serum or plasma, the BD OptEIA™ Mouse IL-4 ELISA Set is recommended (Cat. No. 555198).
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说明
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BD Pharmingen™ Recombinant Mouse IL-4 - Reactivity Ms
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 200 μg/mL
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缓冲液
- Frozen aqueous buffered solution containing BSA and glycerol.
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储存液
- Azide free
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注意事项
- Interleukin-4 (IL-4) is a species-specific cytokine which promotes the proliferation, differentiation and cell-surface protein modulation of B lymphocytes. IL-4 is responsible for activities previously assigned to BCGF, BCDF, BSF-2 and TCGF-2. Mouse IL-4 is a 14 kD protein containing 120 amino acid residues.
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储存条件
- -80 °C
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储存方法
- Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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- IL-4 (IL4) (Interleukin 4 (IL4))
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别名
- IL-4
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背景
- Interleukin-4 (IL-4) is a species-specific cytokine which promotes the proliferation, differentiation and cell-surface protein modulation of B lymphocytes. IL-4 is responsible for activities previously assigned to BCGF, BCDF, BSF-2 and TCGF-2. Mouse IL-4 is a 14 kD protein containing 120 amino acid residues.
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
抗原
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