我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

IL-4 蛋白

Name: IL-4 蛋白
SKU: ABIN1305127
Availability: OutOfStock

This Recombinant IL-4 protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 2+ publications.

(2 references)

产品编号 ABIN1305127

发货至: 中国

Datasheet as PDF

Quick Overview for IL-4 蛋白 (ABIN1305127)

抗原

See all IL-4 (IL4) 蛋白

IL-4 (IL4) (Interleukin 4 (IL4))

蛋白类型

Recombinant

生物活性

Active

宿主

小鼠

资源

大肠杆菌（E. Coli）

应用范围

ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)

纯度

≥ 95 %

Discover our top product IL4 蛋白

品牌

BD Pharmingen™

特异性

Biological Activity: Proliferation is measured using MC/9 indicator cells pulsed with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide).
Specific Activity: 0.3 - 2.0 x 10^8 Units/mg
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.
ED50: 50 - 300 pg/mL

产品特性

Recombinant mouse IL-4 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, pH 7.2, containing glycerol and 2.0 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant mouse IL-4 was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers Lambert Law.

纯化方法

Immunoaffinity Chromatography
Recombinant mouse IL-4 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives. Recombinant mouse IL-4 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

过滤

0.22 μm filtered

内毒素水平

The endotoxin level is ≤ 0.1 ng per µg of mouse IL-4, as measured in a chromogenic LAL assay.
想要此Protein的其他选项吗？

!

探索我们的预定义定制蛋白和定制蛋白服务！

您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案

Interleukin 4 (IL4) (AA 25-153) protein
IL4 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Interleukin 4 (IL4) (AA 25-153) protein (His tag)
IL4 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Interleukin 4 (IL4) (AA 25-153) protein (His-Avi Tag,Biotin)
IL4 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE

Interleukin 4 (IL4) (AA 25-153) protein (His-Avi Tag)
IL4 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE

Interleukin 4 (IL4) (AA 23-140) (Active) protein
IL4 宿主: 小鼠宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active

Interleukin 4 (IL4) (AA 25-153) (Active) protein
IL4 宿主: 人宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active

Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag,AVI tag,Biotin)
IL4 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Interleukin 4 (IL4) protein (His tag)
IL4 宿主: 小鼠宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE

Interleukin 4 (IL4) (AA 25-153) (Active) protein
IL4 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95% by SDS-PAGE SDS, AcA Active

Interleukin 4 (IL4) (AA 63-153) protein (GST tag)
IL4 宿主: 人宿主: 小麦胚 Recombinant WB, ELISA, AA, AP

应用备注

ELISA Standard: Mouse IL-4 is useful as a quantitative standard for measuring mouse IL-4 protein levels in an IL-4 specific sandwich ELISA with the purified 11B11 antibody (Cat. No. 554434) as a capture antibody and the biotinylated BVD6-24G2 (Cat. No. 554390) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse IL-4 standard from ~2,000 to 15 pg/mL should be included in each ELISA plate. For assay of mouse IL-4 in complex fluids such as serum or plasma, the BD OptEIA™ Mouse IL-4 ELISA Set is recommended (Cat. No. 555198).

说明

BD Pharmingen™ Recombinant Mouse IL-4 - Reactivity Ms

限制

仅限研究用
状态

Liquid

浓度

200 μg/mL

缓冲液

Frozen aqueous buffered solution containing BSA and glycerol.

储存液

Azide free

注意事项

Interleukin-4 (IL-4) is a species-specific cytokine which promotes the proliferation, differentiation and cell-surface protein modulation of B lymphocytes. IL-4 is responsible for activities previously assigned to BCGF, BCDF, BSF-2 and TCGF-2. Mouse IL-4 is a 14 kD protein containing 120 amino acid residues.

储存条件

-80 °C

储存方法

Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Paul: "Interleukin-4: a prototypic immunoregulatory lymphokine." in: Blood, Vol. 77, Issue 9, pp. 1859-70, (1991) (PubMed).

Noma, Sideras, Naito, Bergstedt-Lindquist, Azuma, Severinson, Tanabe, Kinashi, Matsuda, Yaoita: "Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter." in: Nature, Vol. 319, Issue 6055, pp. 640-6, (1986) (PubMed).
抗原

IL-4 (IL4) (Interleukin 4 (IL4))

别名

IL-4

背景

Interleukin-4 (IL-4) is a species-specific cytokine which promotes the proliferation, differentiation and cell-surface protein modulation of B lymphocytes. IL-4 is responsible for activities previously assigned to BCGF, BCDF, BSF-2 and TCGF-2. Mouse IL-4 is a 14 kD protein containing 120 amino acid residues.

途径

JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation