(4 references)-
- 抗原 See all IL-2 (IL2) 蛋白
- IL-2 (IL2) (Interleukin 2 (IL2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)
- 品牌
- BD Pharmingen™
- 特异性
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Biological Activity: Activity Range is measured in a proliferation assay using CTLL-2 indicator cells
Specific Activity: 0.1 - 1.0 x 10^9 Units/mg
ED50: 10 - 100 pg/ml
Observed dose response relationship: 80 fold.
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. - 产品特性
- Recombinant mouse IL-2 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing 2.0 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant mouse IL-2 is > 95% pure, as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law.
- 纯化方法
- Recombinant mouse IL-2 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution and bovine serum albumin, with no preservatives. Recombinant mouse IL-2 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.
- 纯度
- ≥ 95 %
- 过滤
- 0.22 μm filtered
- 内毒素水平
- The endotoxin level is ≤ 0.1 ng per μg of mouse IL-2, as measured in a chromogenic LAL assay.
- Top Product
- Discover our top product IL2 蛋白
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Interleukin 2 (IL2) (AA 21-153) protein
IL2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 (IL2) (AA 21-169) protein (His-Avi Tag)IL2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 (IL2) (AA 21-169) protein (His-Avi Tag,Biotin)IL2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 (IL2) (Active) proteinIL2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 2 (IL2) (Active) proteinIL2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Interleukin 2 (IL2) (AA 21-153) (Active) proteinIL2 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Interleukin 2 (IL2) (AA 21-153) (Active) protein (Fc Tag,AVI tag,Biotin)IL2 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 2 (IL2) protein (His tag)IL2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by reducing SDS-PAGE.
Interleukin 2 (IL2) protein (His tag)IL2 宿主: 大鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Interleukin 2 (IL2) (AA 1-153) protein (GST tag)IL2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
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ELISA Standard: Recombinant mouse IL-2 is useful as a quantitative standard for measuring mouse IL-2 protein levels in an IL-2 specific sandwich ELISA with the purified JES6-1A12 antibody (Cat. No. 554424) as a capture antibody and the biotinylated JES6-5H4 antibody (Cat.No. 554426) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse IL-2 standard from ~2,000 to 15 pg/ml should be included in each ELISA plate.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring mouse IL-2 in serum or plasma the mouse IL-2 BD OptEIA™ Set (Cat. No. 555148) is specially formulated and recommended. - 说明
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BD Pharmingen™ Recombinant Mouse IL-2 - Reactivity Ms
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 200 μg/mL
- 缓冲液
- Frozen aqueous buffered solution containing BSA.
- 储存液
- Azide free
- 储存条件
- -80 °C
- 储存方法
- Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
: "Unique structure of murine interleukin-2 as deduced from cloned cDNAs." in: Nature, Vol. 313, Issue 6001, pp. 402-4, (1985) (PubMed).
: "Biochemical separation of interleukin 2." in: Journal of immunological methods, Vol. 39, Issue 3, pp. 185-201, (1981) (PubMed).
: "Interleukin 2: a class of T cell growth factors." in: Immunological reviews, Vol. 51, pp. 257-78, (1981) (PubMed).
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: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
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- 抗原
- IL-2 (IL2) (Interleukin 2 (IL2))
- 别名
- IL-2 (IL2 产品)
- 别名
- IL2 Protein, IL-2 Protein, il2 Protein, TCGF Protein, lymphokine Protein, Il-2 Protein, POIL2 Protein, interleukin 2 Protein, interleukin-15 Protein, IL2 Protein, il2 Protein, LOC100303688 Protein, Il2 Protein
- 背景
- Interleukin-2 (IL-2) is a cytokine which was first described and characterized based upon its potent ability to modulate lymphocyte reactivity and promote long term in vitro culture of antigen-specific effector T lymphocytes. It is responsible for the activities previously ascribed to T cell growth factor, thymocyte stimulating factor, helper-factor and T cell replacing factor, to name a few. IL-2 has also been reported to act on neuronal cells. Mouse IL-2 is a 17.2 kD protein containing 149 amino acid residues.
- 途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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