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IL-10 蛋白

Name: IL-10 蛋白
SKU: ABIN1305072
Availability: OutOfStock

IL10 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant ≥ 95 % ELISA, Func, ICFC, BR Active

(2 references)

产品编号 ABIN1305072

发货至: 中国

Datasheet as PDF

抗原 See all IL-10 (IL10) 蛋白

IL-10 (IL10) (Interleukin 10 (IL10))

蛋白类型

Recombinant

生物活性

Active
宿主
21

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资源
32

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大肠杆菌（E. Coli）

应用范围

ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)

品牌

BD Pharmingen™

特异性

Bioassay: The activity range is measured in a proliferation assay using MC/9 indicator cells. Cells were co-stimulated with recombinant mouse IL-4 (Cat. No. 550067) at 50 pg/ml and were incubated for 24 hours prior to being pulsed. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Specific Activity: 0.5 - 3.3 x 10^7 Units/mg (Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation).
ED50: 0.3 - 2.0 ng/ml
Observed dose response: >250 fold

产品特性

Recombinant human IL-10 is supplied as a frozen, sterile-filtered aqueous buffered solution containing glycerol and biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant human IL-10 was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

纯化方法

Immunoaffinity Chromatography
Recombinant human IL-10 (Cat No. 554611) is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, containing glycerol and bovine serum albumin, with no preservatives. Recombinant human IL-10 is ≥ 95 % pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per μg of human IL-10, as measured in a chromogenic LAL assay.

纯度

≥ 95 %

过滤

0.22 μm filtered

内毒素水平

The endotoxin level is ≤ 0.1 ng per µg of hIL-10, as measured in a chromogenic LAL assay.

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应用备注

ELISA Standard: Recombinant human IL-10 is useful as a quantitative standard for measuring human IL-10 protein levels in an IL-10 specific sandwich ELISA with the purified JES3-9D7 antibody (Cat. No. 554705) as a capture antibody and the biotinylated JES3-12G8 antibody (Cat. 554611) as the detection antibody. To obtain linear standard curves, doubling dilutions of this human IL-10 standard from ~2,000 to 15 pg/ml should be included in each ELISA plate.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring human IL-10 in serum or plasma the BD OptEIA™ Human IL-10 ELISA Set (Cat. No. 555157) and the BD OptEIA™ Human IL-10 ELISA Kit (Cat. No. 550613) are specially formulated and recommended.
Ligand Blocking Control for Immunofluorescent Staining of Cytokines: Recombinant human IL-10 can be used as a blocking control to demonstrate the specificity of IL-10 staining by our conjugated anti-IL-10 antibodies (for example Cat. No. 554498, Cat. No. 554706, Cat. No. 554707).

说明

BD Pharmingen™ Recombinant Human IL-10 - Reactivity Hu

限制

仅限研究用
状态

Liquid

浓度

100 μg/mL

缓冲液

Frozen aqueous buffered solution containing BSA and glycerol.

注意事项

Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.5 kD protein that shares over 80 % sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production. The net effect of IL-10 action appears to be the inhibition of proinflammatory T cell mediated immunity.

储存条件

-80 °C

储存方法

Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Howard, OGarra, Ishida, de Waal Malefyt, de Vries: "Biological properties of interleukin 10." in: Journal of clinical immunology, Vol. 12, Issue 4, pp. 239-47, (1992) (PubMed).

Vieira, de Waal-Malefyt, Dang, Johnson, Kastelein, Fiorentino, deVries, Roncarolo, Mosmann, Moore: "Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 4, pp. 1172-6, (1991) (PubMed).
抗原

IL-10 (IL10) (Interleukin 10 (IL10))

别名

IL-10 (IL10 产品)

别名

CSIF Protein, GVHDS Protein, IL-10 Protein, IL10A Protein, TGIF Protein, Il-10 Protein, IL10X Protein, interleukin 10 Protein, IL10 Protein, Il10 Protein

背景

Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.5 kD protein that shares over 80 % sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production. The net effect of IL-10 action appears to be the inhibition of proinflammatory T cell mediated immunity.

途径

Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints