HMGB1 Protein (Non-oxidizable)
(1 reference)Our Local Distributor
北京 101111
Quick Overview for HMGB1 Protein (Non-oxidizable) (ABIN1176829)
抗原
See all HMGB1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
-
-
产品特性
- Non-oxidizable
-
序列
- MGKGDPKKPR GKMSSYAFFV QTSREEHKKK HPDASVNFSE FSKKSSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFSSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE
-
产品特性
- This product is a mutant protein where all cysteines are replaced with serines. Non-oxidizable chemokine-HMGB1, LPS free, has chemoattractant activity in vitro and in vivo, does not have cytokine-inducing activity and is resistant to inactivation by ROS. This product is produced in E.coli. The product can be used to recruit leukocytes in vivo without inducing cytokine/chemokine production (Venereau et al, 2012). It contains only trace amounts of LPS (<0.4 ng/mg protein), and is tested for the ability y to induce fibroblast migration. LPS free. Measured by its ability to induce migration. Maximal activity in the cell migration assay is obtained at 1 nM.
-
纯化方法
- Purified
-
内毒素水平
- The purified protein is free from LPS (Cambrex Limulus Amoebocyte Assay QCL-1000, <0.4 ng LPS per mg protein).
-
-
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (GST tag)
HMGB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag,Biotin)HMGB1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
High Mobility Group Box 1 (HMGB1) (Active) proteinHMGB1 宿主: 人, 小鼠, 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % Act, CA Active
High Mobility Group Box 1 (HMGB1) (AA 1-90) protein (GST tag)HMGB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
High Mobility Group Box 1 (HMGB1) (Active) protein (Fc Tag)HMGB1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)HMGB1 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS
High Mobility Group Box 1 (HMGB1) (AA 1-215) proteinHMGB1 宿主: 人 宿主: Baculovirus Recombinant > 90 % pure SDS
-
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
缓冲液
- Non-oxidizable chemokine-HMGB1 is lyophilized from 50 mM HEPES buffer, pH 7.9 and 500 mM NaCl.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- The protein once resuspended can be stored frozen (-20°C).
-
-
-
: "Mutually exclusive redox forms of HMGB1 promote cell recruitment or proinflammatory cytokine release." in: The Journal of experimental medicine, Vol. 209, Issue 9, pp. 1519-28, (2012) (PubMed).
-
: "Mutually exclusive redox forms of HMGB1 promote cell recruitment or proinflammatory cytokine release." in: The Journal of experimental medicine, Vol. 209, Issue 9, pp. 1519-28, (2012) (PubMed).
-
- HMGB1 (High Mobility Group Box 1 (HMGB1))
-
别名
- High-mobility group protein B1 (HMGB1)
-
背景
-
HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells. HMGB1 is essential for life: Hmgb1 knockout mice die shortly after birth.HMGB1 contains three conserved cysteine residues (C23, C45 and C106) and is a redox-sensitive. Chemotaxis-HMGB1 (the form with chemoattractanct activity) is completely reduced, cytokine-HMGB1 contains a disulfide bond between C23 and C45, further oxidation to sulfonates abrogate both activities.In Non-oxidizable chemokine-HMGB1 all cysteines are replaced with serines: these replacements preserve chemoattractant activity in vitro and in vivo, eliminate the cytokine-inducing activity and make the protein resistant to inactivation by ROS.
HMGB1 consists of two fairly rigid, L-shaped DNA-binding domains, each referred to as a ‘HMG box’, and an unstructured tail that ends with 30 consecutive negatively charged amino acids. Non-oxidizable chemokine-HMGB1 consists of 215 amino acid residues and has a calculated molecular mass of approximately 24.8 kDa. -
途径
- p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
抗原
-
