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HMGB1 Protein (Non-oxidizable)

Name: HMGB1 Protein (Non-oxidizable)
SKU: ABIN1176829
Availability: OutOfStock

This Recombinant HMGB1 protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 1 publication.

SDS-PAGE with Coomassie Blue staining (HMGB1 Protein (Non-oxidizable))

SDS

Migration assay with 3T3 mouse cells (HMGB1 Protein (Non-oxidizable))

2 images

(1 reference)

产品编号 ABIN1176829

发货至: 中国

Quick Overview for HMGB1 Protein (Non-oxidizable) (ABIN1176829)

抗原

See all HMGB1 蛋白

HMGB1 (High Mobility Group Box 1 (HMGB1))

蛋白类型

Recombinant

生物活性

Active

宿主

人, 小鼠, 大鼠

资源

大肠杆菌（E. Coli）

应用范围

Activation (Act), Cellular Assay (CA)

纯度

> 95 %

Discover our top product HMGB1 蛋白

产品特性

Non-oxidizable

序列

MGKGDPKKPR GKMSSYAFFV QTSREEHKKK HPDASVNFSE FSKKSSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFSSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE

产品特性

This product is a mutant protein where all cysteines are replaced with serines. Non-oxidizable chemokine-HMGB1, LPS free, has chemoattractant activity in vitro and in vivo, does not have cytokine-inducing activity and is resistant to inactivation by ROS. This product is produced in E.coli. The product can be used to recruit leukocytes in vivo without inducing cytokine/chemokine production (Venereau et al, 2012). It contains only trace amounts of LPS (<0.4 ng/mg protein), and is tested for the ability y to induce fibroblast migration. LPS free. Measured by its ability to induce migration. Maximal activity in the cell migration assay is obtained at 1 nM.

纯化方法

Purified

内毒素水平

The purified protein is free from LPS (Cambrex Limulus Amoebocyte Assay QCL-1000, <0.4 ng LPS per mg protein).
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High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (GST tag)
HMGB1 宿主: 人宿主: 小麦胚 Recombinant WB, ELISA, AA, AP

High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)
HMGB1 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag,Biotin)
HMGB1 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

High Mobility Group Box 1 (HMGB1) (Active) protein
HMGB1 宿主: 人, 小鼠, 大鼠宿主: 大肠杆菌（E. Coli） Recombinant > 95 % Act, CA Active

High Mobility Group Box 1 (HMGB1) (AA 1-90) protein (GST tag)
HMGB1 宿主: 人宿主: 小麦胚 Recombinant WB, ELISA, AA, AP

High Mobility Group Box 1 (HMGB1) (AA 1-215) protein
HMGB1 宿主: 人宿主: Baculovirus Recombinant > 90 % pure SDS

High Mobility Group Box 1 (HMGB1) (Active) protein (Fc Tag)
HMGB1 宿主: 小鼠宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active

High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)
HMGB1 宿主: 人宿主: Hi-5 Cells Recombinant > 90 % by SDS - PAGE SDS

High Mobility Group Box 1 (HMGB1) protein
HMGB1 宿主: 人宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE.

High Mobility Group Box 1 (HMGB1) (AA 24-215) protein (His tag)
HMGB1 宿主: 大鼠宿主: 大肠杆菌（E. Coli） Recombinant >90 % (SDS-PAGE)

限制

仅限研究用
状态

Lyophilized

缓冲液

Non-oxidizable chemokine-HMGB1 is lyophilized from 50 mM HEPES buffer, pH 7.9 and 500 mM NaCl.

储存条件

4 °C/-20 °C

储存方法

The protein once resuspended can be stored frozen (-20°C).
Venereau, Casalgrandi, Schiraldi, Antoine, Cattaneo, De Marchis, Liu, Antonelli, Preti, Raeli, Shams, Yang, Varani, Andersson, Tracey, Bachi, Uguccioni, Bianchi: "Mutually exclusive redox forms of HMGB1 promote cell recruitment or proinflammatory cytokine release." in: The Journal of experimental medicine, Vol. 209, Issue 9, pp. 1519-28, (2012) (PubMed).
抗原

HMGB1 (High Mobility Group Box 1 (HMGB1))

别名

High-mobility group protein B1 (HMGB1)

背景

HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells. HMGB1 is essential for life: Hmgb1 knockout mice die shortly after birth.HMGB1 contains three conserved cysteine residues (C23, C45 and C106) and is a redox-sensitive. Chemotaxis-HMGB1 (the form with chemoattractanct activity) is completely reduced, cytokine-HMGB1 contains a disulfide bond between C23 and C45, further oxidation to sulfonates abrogate both activities.In Non-oxidizable chemokine-HMGB1 all cysteines are replaced with serines: these replacements preserve chemoattractant activity in vitro and in vivo, eliminate the cytokine-inducing activity and make the protein resistant to inactivation by ROS.
HMGB1 consists of two fairly rigid, L-shaped DNA-binding domains, each referred to as a ‘HMG box’, and an unstructured tail that ends with 30 consecutive negatively charged amino acids. Non-oxidizable chemokine-HMGB1 consists of 215 amino acid residues and has a calculated molecular mass of approximately 24.8 kDa.

途径

p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome