PykF protein (His tag)
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
1 image
Quick Overview for PykF protein (His tag) (ABIN1098253)
抗原
PykF
蛋白类型
Recombinant
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS)
纯度
> 95 % by SDS - PAGE
-
-
标记
- His tag
-
纯化方法
- purified by chromatography
-
-
-
-
说明
-
Synonyms: pyruvate kinase I, ECK1672, JW1666
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/ml (determined by Bradford assay)
-
缓冲液
- 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
-
储存条件
- 4 °C
-
储存方法
- Avoid repeated freezing and thawing cycles.
-
-
- PykF
-
背景
- PyrF (pyruvate kinase) belongs to the pyruvate kinase family. pykF is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a Magnesium ion. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. Recombinant E.coli pykF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
-
分子量
- 53.3kDa (494aa)
-
NCBI登录号
- NP_416191
抗原
-
You are here:
