PykF protein (His tag)
宿主: E. coli
宿主: 大肠杆菌（E. Coli）
Recombinant
> 95 % by SDS - PAGE
SDS
1 image
-
- 抗原
- PykF
- 蛋白类型
- Recombinant
- 宿主
- E. coli
-
资源
- 大肠杆菌（E. Coli）
- 标记
- His tag
- 应用范围
- SDS-PAGE (SDS)
- 纯化方法
- purified by chromatography
- 纯度
- > 95 % by SDS - PAGE
-
-
- 说明
-
Synonyms: pyruvate kinase I, ECK1672, JW1666
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/ml (determined by Bradford assay)
- 缓冲液
- 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
- 储存条件
- 4 °C
- 储存方法
- Avoid repeated freezing and thawing cycles.
-
- 抗原
- PykF
- 背景
- PyrF (pyruvate kinase) belongs to the pyruvate kinase family. pykF is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a Magnesium ion. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. Recombinant E.coli pykF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 53.3kDa (494aa)
- NCBI登录号
- NP_416191
-
You are here:
