Antigen 85-A protein (His tag)
宿主: Mycobacterium tuberculosis
宿主: Hi-5 Cells
Recombinant
> 90 % by SDS - PAGE
SDS
1 image
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- 抗原
- Antigen 85-A
- 蛋白类型
- Recombinant
- 宿主
- Mycobacterium tuberculosis
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资源
- Hi-5 Cells
- 标记
- His tag
- 应用范围
- SDS-PAGE (SDS)
- 纯化方法
- purified by chromatography
- 纯度
- > 90 % by SDS - PAGE
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Antigen 85-A (AA 53-331), (partial) protein (His tag)
宿主: Mycobacterium tuberculosis 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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- 说明
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Synonyms: Antigen 85 complex A, Mycolytransferase 85A, Fibronectin-binding protein A, TB Ab85A, TBab85A
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/ml (determined by Bradford assay)
- 缓冲液
- 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol
- 储存条件
- 4 °C
- 储存方法
- Avoid repeated freezing and thawing cycles.
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- 抗原
- Antigen 85-A
- 别名
- Antigen 85A
- 背景
- Antigen 85A, belong to the antigen 85 complex (Antigen 85A, B, C). The enzymes of the antigen 85 complex possess mycolyltransferase activity and catalyze the synthesis of the most abundant glycolipid of the mycobacterial cell wall, the cord factor. The cord factor (trehalose 6,6'-dimycolate, TDM) is essential for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6'-monomycolate (TMM) by Antigen 85A. Recombinant Mycobacterium tuberculosis Antigen 85A protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell and purified by using conventional chromatography techniques.
- 分子量
- 32.8kDa (305aa)
- NCBI登录号
- NP_218321
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