Vinculin Protein (VCL) (AA 2-1066)
Quick Overview for Vinculin Protein (VCL) (AA 2-1066) (ABIN1097790)
抗原
See all Vinculin (VCL) 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 2-1066
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原理
- Recombinant Human Vinculin
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序列
- PVFHTRTIES ILEPVAQQIS HLVIMHEEGE VDGKAIPDLT APVAAVQAAV SNLVRVGKET VQTTEDQILK RDMPPAFIKV ENACTKLVQA AQMLQSDPYS VPARDYLIDG SRGILSGTSD LLLTFDEAEV RKIIRVCKGI LEYLTVAEVV ETMEDLVTYT KNLGPGMTKM AKMIDERQQE LTHQEHRVML VNSMNTVKEL LPVLISAMKI FVTTKNSKNQ GIEEALKNRN FTLEKMSAEI NEIIRVLQLT SWDEDAWASK DTEAMKRALA SIDSKLNQAK GWLRDPSASP GDAGEQAIRQ ILDEAGKVGE LCAGKERREI LGTCKMLGQM TDQVADLRAR GQGSSPVAMQ KAQQVSQGLD VLTAKVENAA RKLEAMTNSK QSIAKKIDAA QNWLADPNGG PEGEEQIRGA LAEARKIAEL CDDPKERDDI LRSLGEISAL TSKLADLRRQ GKGDSPEARA LAKQVATALQ NLQTKTNRAV ANSRPAKAAV HLEGKIEQAQ RWIDNPTVDD RGVGQAAIRG LVAEGHRLAN VMMGPYRQDL LAKCDRVDQL TAQLADLAAR GEGESPQARA LASQLQDSLK DLKARMQEAM TQEVSDVFSD TTTPIKLLAV AATAPPDAPN REEVFDERAA NFENHSGKLG ATAEKAAAVG TANKSTVEGI QASVKTAREL TPQVVSAARI LLRNPGNQAA YEHFETMKNQ WIDNVEKMTG LVDEAIDTKS LLDASEEAIK KDLDKCKVAM ANIQPQMLVA GATSIARRAN RILLVAKREV ENSEDPKFRE AVKAASDELS KTISPMVMDA KAVAGNISDP GLQKSFLDSG YRILGAVAKV REAFQPQEPD FPPPPPDLEQ LRLTDELAPP KPPLPEGEVP PPRPPPPEEK DEEFPEQKAG EVINQPMMMA ARQLHDEARK WSSKGNDIIA AAKRMALLMA EMSRLVRGGS GTKRALIQCA KDIAKASDEV TRLAKEVAKQ CTDKRIRTNL LQVCERIPTI STQLKILSTV KATMLGRTNI SDEESEQATE MLVHNAQNLM QSVKETVREA EAASIKIRTD AGFTLRWVRK TPWYQ
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产品特性
- Recombinant Human Vinculin is produced by our E. coli expression system. The target protein is expressed with sequence (Pro2-Gln1066) of Human Vinculin.
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Vinculin (VCL) (AA 2-235) protein (His tag)
VCL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Vinculin (VCL) (AA 777-1066) protein (GST tag)VCL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Vinculin (VCL) (AA 1-1066) protein (GST tag)VCL 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Vinculin (VCL) protein (His tag)VCL 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
Vinculin (VCL) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)VCL 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
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注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. -
有效期
- 3 months
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- Vinculin (VCL)
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别名
- Vinculin
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背景
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Vinculin is a focal adhesion and cytoskeletal protein that distributed mainly at cell-cell junctions and cell-extracellular matrix (ECM) adhesion that belongs to the Vinculin/alpha-Catenin family. Vinculin is an Actin-binding protein and component of the Actin-Linking Functional module that senses and feels the mechanical properties of the extracellular environment. Vinculin is also a key factor that couples, transmits, transduces, and regulates mechanical force between the cytoskeleton and adhesion receptors. Vinculin generally forms two structural states, an open (active) and closed (inactive) state, which are controlled by conformational interaction(s) between the head and tail domains. Vinculin is involved in the mechano-chemical signal transmission of cells by binding to a variety of focal adhesion or cytoskeletal proteins, and plays important roles in cell adhesion, extension, motion, proliferation and survival.
Alternative Names: Vinculin, Metavinculin, VCL -
分子量
- 117 kDa
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UniProt
- P18206
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途径
- Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
抗原
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