Nerve Growth Factor Protein (NGF) (AA 19-241)
This Recombinant Nerve Growth Factor protein is expressed in 大肠杆菌（E. Coli）.
产品编号 ABIN1097406
发货至:
中国
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Quick Overview for Nerve Growth Factor Protein (NGF) (AA 19-241) (ABIN1097406)
抗原
See all Nerve Growth Factor (NGF) 蛋白
Nerve Growth Factor (NGF)
蛋白类型
Recombinant
宿主
人
资源
大肠杆菌（E. Coli）
纯度
> 95 % as determined by reducing SDS-PAGE.
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产品特性
- AA 19-241
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原理
- Recombinant Human pro-Nerve Growth Factor/pro-NGF (Glu19-Ala241)
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序列
- MEPHSESNVP AGHTIPQAHW TKLQHSLDTA LRRARSAPAA AIAARVAGQT RNITVDPRLF KKRRLRSPRV LFSTQPPREA ADTQDLDFEV GGAAPFNRTH RSKRSSSHPI FHRGEFSVCD SVSVWVGDKT TATDIKGKEV MVLGEVNINN SVFKQYFFET KCRDPNPVDS GCRGIDSKHW NSYCTTTHTF VKALTMDGKQ AAWRFIRIDT ACVCVLSRKA VRRA
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产品特性
- Recombinant Human precursor form of Nerve Growth Factor/pro-NGF produced in E. coli is a non-glycosylated non-covalently linked homodimer with each polypeptide chain containing 222 amino acids with an extra N-terminal Met with a molecular mass of 25kDa.
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Nerve Growth Factor (NGF) (AA 122-239) protein
NGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Nerve Growth Factor (NGF) proteinNGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant ≥98 % (HPLC) CVA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.2.
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注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. -
有效期
- 3 months
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- Nerve Growth Factor (NGF)
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背景
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The precursor form of the nerve growth factor (proNGF) like its mature form is characterized by the cystin knot motif consisting of three cystine bridges, whereas proneurotrophins and mature neurotrophins elicit opposite biological effects. ProNGF functions preferentially via the complex of pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin inducing neuronal apoptosis and contributing to age- and disease-related neurodegeneration.
Alternative Names: Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB -
分子量
- 25 kDa
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UniProt
- P01138
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途径
- Regulation of Cell Size
抗原
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